Research support services

IMIBIC Mass Spectrometry and Molecular Imaging Unit (IMSMI)

Proteómica

IMIBIC Mass Spectrometry and Molecular Imaging Unit (IMSMI) provides highly specialised support in the latest mass spectrometry techniques available to those researchers and users who require it. This Unit is specialised in offering services in three of the major omics such as Proteomics, Lipidomics and Metabolomics. In addition, it is particularly specialised in Molecular Imaging (MS-Imaging).

The IMSMI Unit provides researchers with state-of-the-art technologies in the field and offers its services to IMIBIC research groups as well as to the University of Cordoba, Reina Sofia University Hospital, other universities, hospitals and private sector companies.

This Unit has four main lines of work:

  • i) Clinical Proteomics line, consisting of a high-performance liquid chromatograph Evosep-One coupled to mass spectrometry (LC-MS/MS). It provides researchers and users with access to quantitative clinical proteomics analysis in liquid and without labelling (DDA, DIA-PASEF and PRM-PASEF). The studies that can be developed in this line range from small pilot experiments or small populations to large experiments with hundreds of samples and requiring superior speed and quality.
  • ii) Lipidomics line, consisting of a liquid chromatograph coupled to mass spectrometry (LC-MS/MS). It provides a service for the identification and quantification of lipid species in samples in a targeted or untargeted manner at high resolution.
  • iii) Metabolomics line, consisting of a liquid chromatograph coupled to mass spectrometry (LC-MS/MS). It offers a service for the identification and quantification of metabolites in samples in a targeted or untargeted manner at high resolution.
  • iv) Molecular Imaging (MS-Imaging), which provides researchers and users with spatial information on metabolites, lipids, proteins/peptides directly from tissues and biopsies.

The IMSMI Unit provides individualised, fit-for-purpose support to its users, including project planning/experimental design, sample preparation, mass spectrometry analysis and basic and advanced data analysis, including the latest advances available in its area of expertise.

Personnel

Eduardo Chicano Gálvez, PhD
Eduardo Chicano Gálvez, PhD

IMSMI Platform Coordinator. Senior MS-Imaging & Proteomics Specialist

Ángela Peralbo Molina
Ángela Peralbo Molina

Senior Mass Spectrometry Technical Specialist

Ismael Luna Álvarez
Ismael Luna Álvarez

Junior MS Data Analyst

Equipment and Facilities

  • Q-TOF mass spectrometer, TIMSTOF-Pro (Bruker) (Last update: 2021)
  • Q-TOF mass spectrometer, Triple TOF 5600+ (Sciex) (Last update: 2014)
  • Triple quadrupole mass spectrometer, XevoTQS (Waters) (Last update: 2014)
  • MALDI-TOF/TOF Mass spectrometer, 5800 (Sciex) (Last update: 2014)
  • EVOSEP-ONE (Evosep) (Last update: 2021)
  • ELUTE UHPLC (Bruker) (Last update: 2021)
  • nanoHPLC: Eksigent LC400 (Sciex) (Last update: 2014)
  • nanoUPLC: nanoAcquity M-Class (Waters) (Last update: 2014)
  • Sprayer: HTX M5 (HTX Technologies) (Last update: 2021)
  • Sprayer: Sunchrom Suncollect (Sunchrom, Friedrichdorf, Germany) (Last update: 2014)
  • Main programs (and programming languages) used for data analysis: Protein Pilot, Comet and X!Tandem, Proteowizard, TransProteomicsPipeline, MS-Fragger for protein identification; Peak View, Marker View, Skyline, DIANN, MS-Fragger for protein quantification and spectral library generation; Tissue View, MSiReader, Spectralanalysis, Cardinal, SCILS for Maldi Imaging MS. R, Python and Matlab for general analysis purposes (Last update: 2018 - 2021)
  • PaSER: Parallel Search Engine in Real-time (Bruker) (Last update: 2021)
  • Basic laboratory equipment for sample preparation (Last update: 2014)

Services

  • Identification and characterization of proteins by MALDI-TOF/TOF
  • Identification and characterization of proteins by LC-MS/MS: DDA-PASEF
  • Quantitative Proteomics:
    • DIA-PASEF
    • DIA-SWATH
    • PRM-PASEF
    • SRM (Selected Reaction Monitoring, QqQ)
    • Clinical Proteomics analysis using Evosep-One
  • Lipidomics and Metabolomics: Identification, characterization and/or quantification of small molecules in a wide range of sample precedence.
  • MALDI Imaging mass spectrometry (Proteomics/Lipidomics)
  • Project supervision and collaboration
  • Training in software used for data analysis

Relevant Publications

2021

2020

2019

2018

2017

2016

2015