Research Support Services

Services offered by the Proteomics Unit during the Covid-19 lockdown:

During the local restrictions established by Government and Medical Authorities, IMIBIC Proteomics Unit will support scientific community by giving the following online and “on-lab” services:

  • Support in writing of documents to all groups that need proteomics as supporting technology for their future competitive projects or publications.
  • Information regarding technical/methodological questions about proteomics techniques (Experimental planning, data interpretation, data analysis...).
  • MALDI-Imaging Projects and DIA-SWATH Projects: we are able to perform all experimental procedures associated to these workflows in order to advance in your project. If you are interested, please, contact us for scheduling on.
  • We are opened to perform proof-of-concept experiments for the preparation of research proposals for upcoming calls. If you are interested, please, contact us for planification.

To request any of the services mentioned above, please contact us by email at eduardo.chicano@imibic.org.

Proteomics

Proteómica

The Proteomics Unit provides researchers with state-of-the-art technologies in the field. Currently, the Proteomics Unit is a specialised platform in quantitative proteomics and MALDI Imaging (or Molecular Imaging).

The Proteomics Unit offers its analytical services for IMIBIC staff and University of Cordoba and to other universities, hospitals, and private companies.

The Unit has two main state-of-the-art equipment: (i) a high performance liquid chromatography-mass spectrometry platform that provides researchers with access to high-throughput proteomics analyses, ranging from protein identification and characterisation to label-free quantitative proteomics; and (ii) a mass spectrometry-based molecular imaging platform (MALDI-Imaging) that provides spatial information of metabolites, lipids and proteins directly from tissues and biopsies.

The Proteomics Unit provides individualised, fit-for-purpose support including project planning, sample preparation, mass spectrometry analysis, and data analysis

Personnel

Eduardo Chicano Gálvez, PhD
Eduardo Chicano Gálvez, PhD

Proteomics Platform Coordinator. Senior MS-Imaging & Proteomics Specialist

Ismael Luna Álvarez
Ismael Luna Álvarez

Assistant Technician

Cristina Morilla Murga
Cristina Morilla Murga

Assistant Technician

Equipment and Facilities

  • Q-TOF mass spectrometer, Triple TOF 5600+ (Sciex) (Last update: 2014)
  • Triple quadrupole mass spectrometer, XevoTQS (Waters) (Last update: 2014)
  • MALDI-TOF/TOF Mass spectrometer, 5800 (Sciex) (Last update: 2014)
  • nanoHPLC: Eksigent LC400 (Sciex) (Last update: 2014)
  • nanoUPLC: nanoAcquity M-Class (Waters) (Last update: 2014)
  • Sprayer: Sunchrom Suncollect (Sunchrom, Friedrichdorf, Germany) (Last update: 2014)
  • Main programs (and programming languages) used for data analysis: Protein Pilot, Comet and X!Tandem for protein identification; Peak View, Marker View and Skyline for protein quantification; Tissue View, MSiReader, Cardinal for Maldi Imaging MS. R, Python and Matlab for general analysis purposes (Last update: 2018 - 2020)
  • Basic laboratory equipment for sample preparation (Last update: 2014)

Services

  • Identification and characterization of proteins by MALDI-TOF/TOF
  • Identification and characterization of proteins by LC-MS/MS
  • Quantitative Proteomics:
    • SWATH
    • SRM (Selected Reaction Monitoring)
    • pseudoSRM
  • MALDI Imaging mass spectrometry
  • Project supervision and collaboration
  • Training in software used for data analysis

Relevant Publications

2019

2018

2017

2016

2015