Central Support Units for Biomedical Research
3D Bioprinting
The 3D Bioprinting Unit is one of the scientific-technical support units that provides IMIBIC researchers and public or private institutions the necessary resources to conduct their research in the field of bioprinting.
Its main objective is to assist research projects in the creation of biomimetic 3D constructs with applications in regenerative medicine, pharmacology, and biotechnology. We provide scientific and technical advice and support throughout the entire printing process; from the pre-bioprinting phase (development and implementation of 3D bioprinting assays), setup, and establishment of bioprinting parameters to the final phase of characterization and biological evaluation of the bioprinted constructs.
The 3D Bioprinting Unit is fully equipped with a state-of-the-art printer that ensures the safety and viability of bioprinted constructs.
Personnel
Gema García Jurado, PhD
- gema.garcia@imibic.org
- bio3d@imibic.org
- +34 957 213 753 (Corporative 3753)
- +34 609 157 790
Alvaro Carrasco Carmona
Senior Microscopy Technical Specialist
Fundamentals and applications of 3D Bioprinting
3D bioprinting is a process that enables the creation of biological structures layer by layer, using a variety of living cells and biomaterials (bioinks). This precise technique is essential to replicate complex tissues, such as skin, blood vessels, or even entire organs. The success of bioprinting lies in hydrogels, mixtures that mimic the physical characteristics and composition of the extracellular matrix, making them ideal candidates for applications in tissue engineering.
The applications of 3D Bioprinting are very diverse and can be applied in a wide range of fields, including:
Biomedicine
- Cancer: Tumor microenvironment models of cellular vessels, extracellular matrix replicas, cell cultures and organoid cultures.
- Tissue Engineering: Applications related to different tissue types, including epidermal tissue (skin regeneration and drug monitoring studies), vascular, neuronal, muscular, cartilage, and bone tissue.
- Clinical Research: Development of new drugs and therapies. Xeno-free studies discarding animal-derived products.
Pharmaceutical Industry
- Development of organ models for drug screening.
Food Industry
- Creation of complex-structured foods.
Cosmetic and Perfume Industry
- Development of artifical models to test new products.
- Development of personalized skin models.
- Production of synthetic fragances.
Equipment and facilities
REG4LIFE BIOPRINTER (REGEMAT 3D)
- Mechanical extrusion printer
- Biomaterials extrusion from -20 to 100ºC
- Thermoplastic extrusion up to 250ºC
- Biomaterials light-curing by Ultraviolet light 365nm and 405nm
- Syringes with 0.15, 0.58 and 1.20 mm output diameters.
- Real resolution in the X and Y axis (spindel movement) = 150 μm (0.15 mm); Resolution in Z = 400 nm (0.4 microns). Theoretical resolution in X and Y axis = 1.875 microns – 1875 nm
- Extrusion, bicomponent, coaxial, refrigerated, heated and high temperature syringes available.
- Glass plate, Petri dish and multiwell as printing surfaces
- Automatic X, Y and Z calibration
For more information => 3D Bioprinter Technical Specifications
Services
- Scientific consulting and support in 3D bioprinting techniques.
- New 3D bioprinting assays development and implementation
- 3D bioprinting parameters establishment
- Biological validation of functional hydrogels
- Cell viability analysis in bioprinted constructs and cell lines (LIVE/DEAD assay)
- Cytotoxicity analysis in bioprinted constructs and cell lines (XTT assay)
- Morphological characterization of bioprinted constructs (optical and confocal microscopy).
- Absorbance measurements in 96-well plates (colorimetric assays).
For more information => Technical specifications for plate reader.
