Research groups

GC11 Metabolism and Adipocyte Differentiation-Metabolic Syndrome

The research team on Adipobiology is a consolidated research group working on the identification and characterization of novel markers of adipose tissue dysfunction in obesity. Our research is aimed at elucidating the cellular and molecular mechanisms underlying the development of obesity-related comorbidities, including insulin resistance and type 2 diabetes. This research has a two-pronged approach: (a) targeted analyses of the regulation of insulin signaling and lipid storage and mobilization in adipocytes at the cellular and molecular levels, using a wide variety of cellular and molecular methodologies on adipocyte cell lines and primary human adipocytes, and (b) untargeted omics studies.

We employ in vitro models of insulin resistance to mimic different aspects of obesity and metabolic disease, as well as different approaches for the identification of protein networks relevant to adipose tissue function. Likewise, the relevance of impaired organelle function and inter-organelle contacts in obese adipocytes are also explored. Regarding the second approach, we employ several proteomic techniques to identify novel biomarkers and pathways activated and/or impaired in the two major fat depots, subcutaneous and visceral, and its separate components in lean and obese individuals with different degrees of insulin sensitivity. We have also established the protein fingerprint of the adipose tissue in lipodystrophy and after bariatric surgery-induced weight loss. Our omic studies also include the evaluation of the impact of obesity and insulin resistance on the activation and modification (phosphorylation, acetylation) of proteins of the adipose tissue. In collaboration with Dr. Feliciano Priego (GC-21 Metabolomics Identification/quantification of Bioactive Components) and Eduardo Chicano (The Proteomics Research Support Unit of IMIBIC), we have extended our omic studies to the lipidome, including MALDI Imaging, for comparative analyses of the adipose tissue from obese subjects with different degrees of insulin resistance or lipid droplets isolated from adipocytes.

Our current research projects are aimed at characterizing the impact of extracellular matrix composition and remodeling in obesity-associated adipocyte dysfunction and changes in macrophage phenotype in order to decipher novel molecular mechanisms that could pave the way to devise effective therapies in obesity and metabolic disease. Both 2D and 3D culture systems are employed as platforms for testing drugs, sera, or nanotechnology products with the aim of translating research findings to clinical applications.

Research Lines

Networks

    CIBERobn - CIBER Physiopathology of Obesity and Nutrition. National Institute of Health Carlos III.

    Adipoplast - Research Network on Adipose Plasticity and Related Pathologies. Ministry of Economy and Competitiveness, Spain.  

    CA17103 - COST Action “Delivery of Antisense RNA Therapeutics”

    PAIDI BIO-139 - Cellular and molcular endocrinology

    ADIPOSEQ. CIBERobn, National Institute of Health Carlos III.

     

Keywords

  • adipose tissue
  • adipocytes
  • adipogenesis
  • bariatric surgery
  • cellular stress
  • extracellular matrix
  • fibrosis
  • cell imaging
  • insulin signaling/insulin resistance
  • intracellular traffic
  • lipid droplets
  • lipid metabolism
  • microscopy
  • obesity
  • proteomics
  • protein networks
Dra. María del Mar Malagón Poyato

Principal Investigator

Projects

PROJECTS IN 2020

Ongoing projects

National

María del Mar Malagón Poyato; Caracterización del matrisoma del tejido adiposo y papel de la remodelación de la matriz extracelular en la resistencia a insulina asociada a obesidad; Funding Agency: AGENCIA ESTATAL DE INVESTIGACIÓN; Reference: PID2019-108403RB-I00

PROJECTS IN 2019

Ongoing projects

Regional

Guzmán Ruiz, R. Identification of biomarkers predictive of response to bariatric surgery in morbidly obese patients. Advanced proteomics techniques and applications. Funding agency: Regional Ministry of Equality, Health and Social Policies (CISPS). Reference: PI-0159-2016

National

Malagón, MM (node Córdoba), Villarroya, F. (PI). Research network on adipose plasticity pathophysiologic impact. Funding Agency: Spanish Ministry of Economy and Competitiveness (MINECO). Reference: BFU2015-70454-REDT

Malagón, MM. Characterization of extracellular and intracellular constituents that define expensed adipose tissue. Funding Agency: Spanish Ministry of Economy and Competitiveness (MINECO).  Reference: BFU2016-76711-R 

Gordon, A. Exploring cell communication pathways in the adipose tissue for novel nanomedicine applications in obesity. Funding Agency: Spanish Ministry of Science, Innovation and Universities (MINECO).  Reference: RTI2018-095736-J-100

Clemente-Postigo M. Deciphering insulin resistance in obesity: clues from adipose tissue fibrosis. Juan de la Cierva Formación. Funding Agency: Spanish Ministry of Science, Innovation and Universities (MINECO). Reference: FJCI-2017-32194

Publications

Publications in 2020

Camargo A, Vals-Delgado C, Alcala-Diaz JF, Villasanta-Gonzalez A, Gomez-Delgado F, Haro C, Leon-Acuna A, Cardelo MP, Torres-Pena JD, Guler I, Malagon MM, Ordovas JM, Perez-Martinez P, Delgado-Lista J, Lopez-Miranda J. A Diet-Dependent Microbiota Profile Associated with Incident Type 2 Diabetes: From the CORDIOPREV Study. MOLECULAR NUTRITION & FOOD RESEARCH. 2020. 64 (23):- DOI: 10.1002/mnfr.202000730 
IF: 5,309 Q: 1 D: 1

Navarro-Ruiz MC, Cayuela A, Soriano ML, Guzman-Ruiz R, Malagon MM, Valcarcel M. A Systematic Comparative Study of the Toxicity of Semiconductor and Graphitic Carbon-Based Quantum Dots Using In Vitro Cell Models. APPLIED SCIENCES-BASEL. 2020. 10 (24):- DOI: 10.3390/app10248845 
IF: 2,474 Q: 2

Guzman-Ruiz R, Tercero-Alcazar C, Rabanal-Ruiz Y, Diaz-Ruiz A, El Bekay R, Rangel-Zuniga OA, Navarro-Ruiz MC, Molero L, Membrives A, Ruiz-Rabelo JF, Pandit A, Lopez-Miranda J, Tinahones FJ, Malagon MM. Adipose tissue depot-specific intracellular and extracellular cues contributing to insulin resistance in obese individuals. FASEB JOURNAL. 2020. 34 (6):7520-7539 DOI: 10.1096/fj.201902703R 
IF: 4,966 Q: 1 D: 1

Fruhbeck G, Balaguer I, Mendez-Gimenez L, Valenti V, Becerril S, Catalan V, Gomez-Ambrosi J, Silva C, Salvador J, Calamita G, Malagon MM, Rodriguez A. Aquaporin-11 Contributes to TGF-beta 1-induced Endoplasmic Reticulum Stress in Human Visceral Adipocytes: Role in Obesity-Associated Inflammation. CELLS. 2020. 9 (6):- DOI: 10.3390/cells9061403 
IF: 4,366 Q: 2

Boughanem H, Cabrera-Mulero A, Hernandez-Alonso P, Clemente-Postigo M, Casanueva FF, Tinahones FJ, Morcillo S, Crujeiras AB, Macias-Gonzalez M. Association between variation of circulating 25-OH vitamin D and methylation of secreted frizzled-related protein 2 in colorectal cancer. CLINICAL EPIGENETICS. 2020. 12 (1):- DOI: 10.1186/s13148-020-00875-9 
IF: 5,028 Q: 1

Ortiz-Morales AM, Alcala-Diaz JF, Rangel-Zuniga OA, Corina A, Quintana-Navarro G, Cardelo MP, Yubero-Serrano E, Malagon MM, Delgado-Lista J, Ordovas JM, Lopez-Miranda J, Perez-Martinez P. Biological senescence risk score. A practical tool to predict biological senescence status. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION. 2020. 50 (11):- DOI: 10.1111/eci.13305 
IF: 3,481 Q: 1

Vohl MC, Malagon MM, Ramos-Molina B. Editorial: Dietary Factors, Epigenetics and Their Implications for Human Obesity. FRONTIERS IN ENDOCRINOLOGY. 2020. 11 ():- DOI: 10.3389/fendo.2020.00601 
IF: 3,644 Q: 2

de la Cruz-Ares S, Cardelo MP, Gutierrez-Mariscal FM, Torres-Pena JD, Garcia-Rios A, Katsiki N, Malagon MM, Lopez-Miranda J, Perez-Martinez P, Yubero-Serrano EM. Endothelial Dysfunction and Advanced Glycation End Products in Patients with Newly Diagnosed Versus Established Diabetes: From the CORDIOPREV Study. NUTRIENTS. 2020. 12 (1):- DOI: 10.3390/nu12010238 
IF: 4,546 Q: 1

Lecube A, Sanchez E, Monereo S, Medina-Gomez G, Bellido D, Garcia-Almeida JM, de Icaya PM, Malagon MM, Goday A, Tinahones FJ. Factors Accounting for Obesity and Its Perception among the Adult Spanish Population: Data from 1,000 Computer-Assisted Telephone Interviews. OBESITY FACTS. 2020. 13 (4):322-332 DOI: 10.1159/000508111 
IF: 3,514 Q: 2

Martin-Nunez GM, Cornejo-Pareja I, Roca-Rodriguez MD, Clemente-Postigo M, Cardona F, Fernandez-Garcia JC, Moreno-Indias I, Tinahones FJ. H. pylori Eradication Treatment Causes Alterations in the Gut Microbiota and Blood Lipid Levels. FRONTIERS IN MEDICINE. 2020. 7 ():- DOI: 10.3389/fmed.2020.00417 
IF: 3,9 Q: 1

Gomes A, Soares R, Costa R, Marino F, Cosentino M, Malagon MM, Ribeiro L. High-fat diet promotes adrenaline production by visceral adipocytes. EUROPEAN JOURNAL OF NUTRITION. 2020. 59 (3):1105-1114 DOI: 10.1007/s00394-019-01971-0 
IF: 4,664 Q: 1

Fernandez-Vega A, Chicano-Galvez E, Prentice BM, Anderson D, Priego-Capote F, Lopez-Bascon MA, Calderon-Santiago M, Avendano MS, Guzman-Ruiz R, Tena-Sempere M, Fernandez JA, Caprioli RM, Malagon MM. Optimization of a MALDI-Imaging protocol for studying adipose tissue-associated disorders. TALANTA. 2020. 219 ():- DOI: 10.1016/j.talanta.2020.121184 
IF: 5,339 Q: 1

Aranda-Valera IC, de la Rosa IA, Roldan-Molina R, Abalos-Aguilera MD, Torres-Granados C, Patino-Trives A, Luque-Tevar M, Ibanez-Costa A, Guzman-Ruiz R, Malagon MD, Escudero-Contreras A, Lopez-Pedrera C, Collantes-Estevez E, Barbarroja N. Subclinical cardiovascular risk signs in adults with juvenile idiopathic arthritis in sustained remission. PEDIATRIC RHEUMATOLOGY. 2020. 18 (1):- DOI: 10.1186/s12969-020-00448-3 
IF: 2,595 Q: 1

Clemente-Postigo M, Tinahones A, El Bekay R, Malagon MM, Tinahones FJ. The Role of Autophagy in White Adipose Tissue Function: Implications for Metabolic Health. METABOLITES. 2020. 10 (5):- DOI: 10.3390/metabo10050179 
IF: 4,097 Q: 2

Publications in 2019

Ortega FJ, Moreno-Navarrete JM, Mercader JM, Gómez-Serrano M, García-Santos E, Latorre J, Lluch A, Sabater M, Caballano-Infantes E, Guzmán R, Macías-González M, Buxo M, Gironés J, Vilallonga R, Naon D, Botas P, Delgado E, Corella D, Burcelin R, Frühbeck G, Ricart W, Simó R, Castrillon-Rodríguez I, Tinahones FJ, Bosch F, Vidal-Puig A, Malagón MM, Peral B, Zorzano A, Fernández-Real JM. Cytoskeletal transgelin 2 contributes to gender-dependent adipose tissue expandability and immune function. FASEB J. 2019 Aug;33(8):9656-9671. doi: 10.1096/fj.201900479R.
IF: 5,391 Q: 1 D: 1

Gomes A, Soares R, Costa R, Marino F, Cosentino M, Malagon MM, Ribeiro L. High-fat diet promotes adrenaline production by visceral adipocytes. Eur J Nutr. 2019 Apr 22. doi: 10.1007/s00394-019-01971-0.
IF: 4,449 Q: 1

Gousset K, Gordon A, Kumar Kannan S, Tovar J. A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions. Int J Mol Sci. 2019 Mar 7;20(5). pii: E1172. doi: 10.3390/ijms20051172.
IF: 4,183 Q: 1

Uhl J, Gujarathi S, Waheed AA, Gordon A, Freed EO, Gousset K. Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes.  J Cell Commun Signal. 2019 Jun;13(2):209-224. doi: 10.1007/s12079-018-0493-z.
IF: 3,691 Q: 2

Clemente-Postigo M, Oliva-Olivera W, Coin-Aragüez L, Ramos-Molina B, Giraldez-Perez RM, Lhamyani S, Alcaide-Torres J, Perez-Martinez P, El Bekay R, Cardona F, Tinahones FJ. Metabolic endotoxemia promotes adipose dysfunction and inflammation in human obesity. Am J Physiol Endocrinol Metab. 2019;316(2): E319-E332. doi: 10.1152/ajpendo.00277.2018.
IF: 4,125 Q: 1

Gentile AM, Lhamyani S, Coín-Aragüez L, Clemente-Postigo M, Oliva Olivera W, Romero-Zerbo SY, García-Serrano S, García-Escobar E, Zayed H, Doblado E, Bermúdez-Silva FJ, Murri M, Tinahones FJ, El Bekay R. miR-20b, miR-296, and Let-7f Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes. Obesity (Silver Spring). 2019;27(2):245-254. doi: 10.1002/oby.22363.
IF: 3,969 Q: 1

Cornejo-Pareja I, Clemente-Postigo M*, Tinahones FJ. Metabolic and Endocrine Consequences of Bariatric Surgery. Front Endocrinol (Lausanne). 2019; 10:626. doi: 10.3389/fendo.2019.00626. *Corresponding author.
IF: 3,634 Q: 2

Publications in 2018

Travez A, Rabanal-Ruiz Y, Lopez-Alcala J, Molero-Murillo L, Diaz-Ruiz A, Guzman-Ruiz R, Catalan V, Rodriguez A, Fruhbeck G, Tinahones FJ, Gasman S, Vitale N, Jimenez-Gomez Y, Malagon MM. The caveolae-associated coiled-coil protein, NECC2, regulates insulin signalling in Adipocytes. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2018; 22(11):5648-5661.
IF: 4,302 Q: 1

Quinones M, Al-Massadi O, Folgueira C, Bremser S, Gallego R, Torres-Leal L, Haddad-Tovolli R, Garcia-Caceres C, Hernandez-Bautista R, Lam BYH, Beiroa D, Sanchez-Rebordelo E, Senra A, Malagon JA, Valerio P, Fondevila MF, Ferno J, Malagon MM, Contreras R, Pfluger P, Bruning JC, Yeo G, Tschop M, Dieguez C, Lopez M, Claret M, Kloppenburg P, Sabio G, Nogueiras R. p53 in AgRP neurons is required for protection against diet-induced obesity via JNK1. NATURE COMMUNICATIONS. 2018; 9:Núm artículo 3432
IF: 12,353 Q: 1 D: 1

de la Rosa IA, Escudero-Contreras A, Rodriguez-Cuenca S, Ruiz-Ponce M, Jimenez-Gomez Y, Ruiz-Limon P, Perez-Sanchez C, Abalos-Aguilera MC, Cecchi I, Ortega R, Calvo J, Guzman-Ruiz R, Malagon MM, Collantes-Estevez E, Vidal-Puig A, Lopez-Pedrera C, Barbarroja N. Defective glucose and lipid metabolism in rheumatoid arthritis is determined by chronic inflammation in metabolic tissues. JOURNAL OF INTERNAL MEDICINE. 2018; 284(1):61-77.
IF: 6,754 Q: 1 D: 1

Gomez-Marin B, Gomez-Delgado F, Lopez-Moreno J, Alcala-Diaz JF, Jimenez-Lucena R, Torres-Pena JD, Garcia-Rios A, Ortiz-Morales AM, Yubero-Serrano EM, Malagon MD, Lai CQ, Delgado-Lista J, Ordovas JM, Lopez-Miranda J, Perez-Martinez P. Long-term consumption of a Mediterranean diet improves postprandial lipemia in patients with type 2 diabetes: the Cordioprev randomized trial. AMERICAN JOURNAL OF CLINICAL NUTRITION. 2018; 108(5):963-970.
IF: 6,549 Q: 1 D: 1

Roncero-Ramos I, Jimenez-Lucena R, Alcala-Diaz JF, Vals-Delgado C, Arenas-Larriva AP, Rangel-Zuniga OA, Leon-Acuna A, Malagon MM, Delgado-Lista J, Perez-Martinez P, Ordovas JM, Camargo A, Lopez-Miranda J. Alpha cell function interacts with diet to modulate prediabetes and Type 2 diabetes. JOURNAL OF NUTRITIONAL BIOCHEMISTRY. 2018; 62:47-256.
IF: 4,414 Q: 1

Lopez-Bascon MA, Calderon-Santiago M, Sanchez-Ceinos J, Fernandez-Vega A, Guzman-Ruiz R, Lopez-Miranda J, Malagon MM, Priego-Capote F. Influence of sample preparation on lipidomics analysis of polar lipids in adipose tissue. TALANTA. 2018; 177:86-93.
IF: 4,244 Q: 1

Santos-Marcos, JA; Rangel-Zuniga, OA; Jimenez-Lucena, R; Quintana-Navarro, GM; Garcia-Carpintero, S; Malagon, MM; Landa, BB; Tena-Sempere, M; Perez-Martinez, P; Lopez-Miranda, J; Perez-Jimenez, F; Camargo, A. Influence of gender and menopausal status on gut microbiota. MATURITAS. 2018; 116:43-53.
IF: 3,315 Q: 1

Lopez-Moreno J, Garcia-Carpintero S, Gomez-Delgado F, Jimenez-Lucena R, Vals-Delgado C, Alcala-Diaz JF, Roncero-Ramos I, Rangel-Zuniga OA, Yubero-Serrano EM, Malagon MM, Ordovas JM, Perez-Martinez P, Lopez-Miranda J, Camargo A. Endotoxemia is modulated by quantity and quality of dietary fat in older adults. EXPERIMENTAL GERONTOLOGY. 2018; 109:119-125.
IF: 3,224 Q: 2

Publications in 2017

Moreno-Castellanos N, Rodriguez A, Rabanal-Ruiz Y, Fernandez-Vega A, Lopez-Miranda J, Vazquez-Martinez R, Fruhbeck G, Malagon M. The cytoskeletal protein septin 11 is associated with human obesity and is involved in adipocyte lipid storage and metabolism. DIABETOLOGIA. 2017.60(2):324-335.
IF: 6,08 Q: 1 

Martinez-Sanchez N, Seoane-Collazo P, Contreras C, Varela L, Villarroya J, Rial-Pensado E, Buque X, Aurrekoetxea I, Delgado TC, Vazquez-Martinez R, Gonzalez-Garcia I, Roa J, Whittle AJ, Gomez-Santos B, Velagapudi V, Tung YCL, Morgan DA, Voshol PJ, de Morentin PBM, Lopez-Gonzalez T, Linares-Pose L, Gonzalez F, Chatterjee K, Sobrino T, Medina-Gomez G, Davis RJ, Casals N, Oresic M, Coll AP, Vidal-Puig A, Mittag J, Tena-Sempere M, Malagon MM, Dieguez C, Martinez-Chantar ML, Aspichueta P, Rahmouni K, Nogueiras R, Sabio G, Villarroya F, Lopez M. Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance. CELL METABOLISM. 2017.26(1):212-229.e12.
IF: 18,164 Q: 1  D: 1

Porteiro B, Fondevila MF, Delgado TC, Iglesias C, Imbernon M, Iruzubieta P, Crespo J, Zabala-Letona A, Ferno J, Gonzalez-Teran B, Matesanz N, Hernandez-Cosido L, Marcos M, Tovar S, Vidal A, Sanchez-Ceinos J, Malagon MM, Pombo C, Zalvide J, Carracedo A, Buque X, Dieguez C, Sabio G, Lopez M, Aspichueta P, Martinez-Chantar ML, Nogueiras R. Hepatic p63 regulates steatosis via IKK beta/ER stress. NATURE COMMUNICATIONS. 2017.8():-15111.
IF: 12,124 Q: 1  D: 1

Mendez-Gimenez L, Becerril S, Moncada R, Valenti V, Fernandez S, Ramirez B, Catalan V, Gomez-Ambrosi J, Soveral G, Malagon MM, Dieguez C, Rodriguez A, Fruhbeck G. Gastric Plication Improves Glycemia Partly by Restoring the Altered Expression of Aquaglyceroporins in Adipose Tissue and the Liver in Obese Rats. OBESITY SURGERY. 2017.27(7):1763-1774.
IF: 3,947 Q: 1  D: 1

Novelle MG, Vazquez MJ, Peinado JR, Martinello KD, Lopez M, Luckman SM, Tena-Sempere M, Malagon MM, Nogueiras R, Dieguez C.Sequential Exposure to Obesogenic Factors in Females Rats: From Physiological Changes to Lipid Metabolism in Liver and Mesenteric Adipose Tissue.  SCIENTIFIC REPORTS. 2017.7:-46194.
IF: 4,259 Q: 1

Sarmento-Cabral A, Peinado JR, Halliday LC, Malagon MM, Castaño JP, Kineman RD, Luque RM. Adipokines (Leptin, Adiponectin, Resistin) Differentially Regulate All Hormonal Cell Types in Primary Anterior Pituitary Cell Cultures from Two Primate Species. SCIENTIFIC REPORTS. 2017;7:43537.
IF: 4,259 Q: 1

Lopez-Moreno J, Garcia-Carpintero S, Gomez-Delgado F, Jimenez-Lucena R, Vals-Delgado C, Alcala-Diaz JF, Roncero-Ramos I, Rangel-Zuñiga OA, Yubero-Serrano EM, Malagon MM, Ordovas JM, Perez-Martinez P, Lopez-Miranda J, Camargo A. Endotoxemia is modulated by quantity and quality of dietary fat in older adults. Experimental gerontology. 2017. Nov 10. pii: S0531-5565(17)30652-6
IF: 3,34 Q: 1 

Members

Principal Investigator
María del Mar Malagón Poyato

Researchers
Ana Gordón Bermúdez-Coronel
Rocío Guzmán Ruiz

Post-Doctoral Researchers
María Mercedes Clemente Postigo

Pre-Doctoral Researchers
Jaime López Alcalá
María del Carmen Ruiz Navarro
Julia Sánchez Ceinos
Carmen Tercero Alcázar

Nursing, Technical, and Administrative Staff
Laura Molero Murillo