Research groups

GC21 Metabolomics and identification of bioactive compounds

Our group is mid-size team formed by 10 people. It is a consolidated group that is dedicated to basic transversal research. Our main scientific activity is focused on the development and application of metabolomics methods for application in clinical and agrofood areas.

We are specialized in targeted and untargeted metabolomics analysis by using mass spectrometry. For this purpose, we are well equipped with GC-MS and LC-MS/MS equipment both in high and low resolution acquisition modes to be implemented in metabolomics analysis. In targeted analysis our main objective is to enhance sensitivity and selectivity as well as to obtain a high automation level. In this context, we have contributed to the development of a number of analytical methods with applicability in clinical analysis (vitamin D and metabolites, lipid inflammation markers, phospholipids, etc.) and agrofood characterization (phenolic compounds in olive oil, steviol glycosides, etc.). Concerning untargeted analysis our aim is to combine analytical methods to maximize the number of detected metabolites in a specific sample.

We also develop analytical methods for characterization of biological samples with special emphasis on biofluids and tissues. Finally, a pipeline for identification, configuration of biomarkers panels, and further validation is also available to be applied in metabolomics analysis.

Due to our transversal focus we collaborate with clinical groups to offer a complementary view to be implemented in biomedical studies. Additionally, our group is active in the development of research projects with public and private companies which take benefits from the methodological innovation proposed by our group.

Research Lines

Networks

Keywords

  • metabolomics
  • mass spectrometry
  • targeted analysis
  • untargeted analysis
  • sample preparation
  • clinical analysis
  • agrofood
  • bioactive compounds

GC21 Metabolomics and identification of bioactive compounds

Principal Investigator
Feliciano Priego Capote

Co-Principal Investigator
María Dolores Luque de Castro

Post-Doctoral Researchers
Mónica Calderón Santiago
Carlos Augusto Ledesma Escobar
Mª del Mar Delgado Povedano
Mª Asunción López Bascón

Pre-Doctoral Researchers
Azahara Díaz Lozano
Laura de Los Santos Castillo Peinado
Diego Luque Córdoba
Antonio Mena Bravo
Inmaculada Criado Navarro

Projects

Active projects

National

Priego Capote, F. Strategy development for improving the mass-spectrometric detection in guided metabiological analysis and global applied to clinical and nutrition. Funding agency: Spanish Ministry of Economy and Competitiveness (MINECO). Reference: CTQ2015-68813-R.

Caracterización de las fracciones funcionales de la aceituna, formulación de nuevos productos y análisis de sus efectos: NUTRADAF. Funding agency: CDTI Feder Interconecta. Reference: ITC-20161265.

International

Priego Capote, F. Aristoil Project. Funging agency: European Commission, Interreg-Med Program. Reference: MED-1033

Finished projects

Desarrollo de grasas avanzadas GR2020. Funding agency: CDTI Feder Interconecta. Reference: ITC-20151323.

Publications

Publications 2019

Criado-Navarro I, Mena-Bravo A, Calderon-Santiago M, Priego-Capote F; Determination of glycerophospholipids in vegetable edible oils: Proof of concept to discriminate olive oil categories. FOOD CHEMISTRY. 2019 Nov 30;299:125136. doi: 10.1016/j.foodchem.2019.125136.
IF: 5,399 
D: 1

Fernandez-Bedmar Z, Anter J, Alonso-Moraga A, de la Torre PD, Luque de Castro MD, Millan-Ruiz Y, Sanchez-Frias M, Guil-Luna S; Red and White Wine Lees Show Inhibitory Effects on Liver Carcinogenesis. MOLECULAR NUTRITION & FOOD RESEARCH. 2019 May;63(9):e1800864. doi: 10.1002/mnfr.201800864. 
IF: 5,151
D: 1

Lopez-Bascon MA, Calderon-Santiago M, Arguello H, Morera L, Garrido JJ, Priego-Capote F; Comprehensive analysis of pig feces metabolome by chromatographic techniques coupled to mass spectrometry in high resolution mode: Influence of sample preparation on the identification coverage. TALANTA. 2019 Jul 1;199:303-309. doi: 10.1016/j.talanta.2019.02.073. 
IF: 4,916
Q: 1

Castillo-Peinado LS, Lopez-Bascon MA, Mena-Bravo A, Luque de Castro MD, Priego-Capote F; Determination of primary fatty acid amides in different biological fluids by LC-MS/MS in MRM mode with synthetic deuterated standards: Influence of biofluid matrix on sample preparation. TALANTA. 2019 Feb 1;193:29-36. doi: 10.1016/j.talanta.2018.09.088.
IF: 4,916
Q: 1

Mena-Bravo A, Calderon-Santiago M, Luque de Castro MD, Priego-Capote F; Evaluation of short-term storage prior to analysis of vitamin D-3 and metabolites in human serum by liquid chromatography coupled to tandem mass spectrometry. TALANTA. 2019 Jun 1;198:344-349. doi: 10.1016/j.talanta.2019.02.030. 
IF: 4,916
Q: 1

Luque de Castro MD, Jurado-Lopez A; The role of analytical chemists in the research on the cultural heritage. TALANTA. 2019 Dec 1;205:120106. doi: 10.1016/j.talanta.2019.07.001.
IF: 4,916

Brito RE, Priego-Capote F, Ledesma-Escobar C, Montoya MR, Mellado JMR; Electrochemical Oxidation Pathways of Hydroxycoumarins on Carbon Electrodes Examined by LSCV and LC-MS/MS. JOURNAL OF THE ELECTROCHEMICAL SOCIETY. 2019. 166(8):H331-H335. doi: 10.1149/2.0781908jes
IF: 3,12

Ledesma-Escobar CA, Priego-Capote F, Robles-Olvera VJ, Garcia-Torres R, De Corcuera JIR, de Castro MDL; GC-MS study of changes in polar/mid-polar and volatile compounds in Persian lime (Citrus latifolia) during fruit growth. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE. 2019 Feb;99(3):1020-1028. doi: 10.1002/jsfa.9266.
IF: 2,422
Q: 1 

Lope V, Toribio MJ, Perez-Gomez B, Castello A, Mena-Bravo A, Sierra MA, Lucas P, Herran-Vidaurrazaga MD, Gonzalez-Vizcayno C, Pino MN, Cruz-Campos I, Roca-Navarro MJ, Aragones N, Romieu I, Martinez-Cortes M, de Castro MDL, Pollan M; Serum 25-hydroxyvitamin D and mammographic density in premenopausal Spanish women. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2019 May;189:101-107. doi: 10.1016/j.jsbmb.2019.03.004.
IF: 3,785
Q: 2

Delgado-Povedano MM, de Castro MDL; The 'in medium virtus' assessment of green analytical chemistry. CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY. 19. 8-14. SI. doi: 10.1016/j.cogsc.2019.02.008. 
IF: -

Main Publications 2018

Miho H, Diez CM, Mena-Bravo A, de Medina VS, Moral J, Melliou E, Magiatis P, Rallo L, Barranco D, Priego-Capote F. Cultivar influence on variability in olive oil phenolic profiles determined through an extensive germplasm survey. FOOD CHEMISTRY. 2018; 266:192-199.
IF: 4,946  
Q: 1   D: 1

Ledesma-Escobar CA, Priego-Capote F, Robles-Olvera VJ, Luque de Castro MD. Targeted analysis of the concentration changes of phenolic compounds in Persian lime (Citrus latifolia) during fruit growth. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2018; 66(8):1813-1820.
IF: 3,412  
Q: 1   D: 1

Delgado-Povedano MM, Calderon-Santiago M, Luque de Castro MD, Priego-Capote F. Metabolomics analysis of human sweat collected after moderate exercise. TALANTA. 2018; 177:47-65.
IF: 4,244  
Q: 1

Lopez-Bascon MA, Calderon-Santiago M, Sanchez-Ceinos J, Fernandez-Vega A, Guzman-Ruiz R, Lopez-Miranda J, Malagon MM, Priego-Capote F. Influence of sample preparation on lipidomics analysis of polar lipids in adipose tissue. TALANTA 2018; 177:86-93.
IF: 4,244  
Q: 1

Luque-Cordoba D, Calderon-Santiago M, Luque de Castro MD, Priego-Capote F. Study of sample preparation for determination of endocannabinoids and analogous compounds in human serum by LC-MS/MS in MRM mode. TALANTA 2018; 185:602-610.
IF: 4,244  
Q: 1

Luque de Castro MD, Priego-Capote F. The analytical process to search for metabolomics biomarkers. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. 2018; 147:341-349.
IF: 2,831  
Q: 2

Other Publications 2018

Tsolakou A, Diamantakos IP, Kalaboki I, Mena-Bravo A, Priego-Capote F, Abdallah IM, Kaddoumi A, Melliou E, Magiatis P. Oleocanthalic Acid, a Chemical Marker of Olive Oil Aging and Exposure to a High Storage Temperature with Potential Neuroprotective Activity. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2018; 66(28):7337-7346.
IF: 3,412  
Q: 1   D: 1

Moreno P, Jimenez-Jimenez C, Garrido-Rodriguez M, Calderon-Santiago M, Molina S, Lara-Chica M, Priego-Capote F, Salvatierra A, Munoz E, Calzado MA. Metabolomic profiling of human lung tumor tissues - nucleotide metabolism as a candidate for therapeutic interventions and biomarkers. MOLECULAR ONCOLOGY. 2018; 12(10):1778-1796.
IF: 5,264  
Q: 1

Arguello H, Estelle J, Zaldivar-Lopez S, Jimenez-Marin A, Carvajal A, Lopez-Bascon MA, Crispie F, O'Sullivan O, Cotter PD, Priego-Capote F, Morera L, Garrido JJ. Early Salmonella Typhimurium infection in pigs disrupts Microbiome composition and functionality principally at the ileum mucosa. SCIENTIFIC REPORTS. 2018; 8: Núm artículo 7788.
IF: 4,122  
Q: 1

Lope V, Castello A, Mena-Bravo A, Amiano P, Aragones N, Fernandez-Villa T, Guevara M, Dierssen-Sotos T, Fernandez-Tardon G, Castano-Vinyals G, Marcos-Gragera R, Moreno V, Salas-Trejo D, Diaz-Santos M, Oribe M, Romieu I, Kogevinas M, Priego-Capote F, Perez-Gomez B, Pollan M. Serum 25-hydroxyvitamin D and breast cancer risk by pathological subtype (MCC-Spain). JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2018; 182:4-13.
IF: 4,095  
Q: 1

Rallo L, Diez CM, Morales-Sillero A, Miho H, Priego-Capote F, Rallo P. Quality of olives: A focus on agricultural preharvest factors. SCIENTIA HORTICULTURAE. 2018; 233:491-509.
IF: 1,76  
Q: 1

Garcia-Valverde MT, Ledesma-Escobar CA, Lucena R, Cardenas S. Tunable Polarity Carbon Fibers, a Holistic Approach to Environmental Protection. MOLECULES. 2018; 23(5): Núm artículo 1026.
IF: 3,098  
Q: 2

Jimenez-Munguia I, Calderon-Santiago M, Rodriguez-Franco A, Priego-Capote F, Rodriguez-Ortega MJ. Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4. PEERJ. 2018; 6: Núm artículo e4966.
IF: 2,118  
Q: 2

Main Publications 2017

Ledesma-Escobar C.A, Priego-Capote F, Robles-Olvera VJ, Luque de Castro MD. Changes in the composition of the polar fraction of Persian lime (Citrus latifolia) during fruit growth by LC–QTOF MS/MS analysis. Food Chemistry 2017.234:262-268.
IF: 4,529
Q: 1  D: 1

Delgado-Povedano MD, Priego-Capote F, de Castro M.D. Selective ultrasound-enhanced enzymatic hydrolysis of oleuropein to its aglycon in olive (Olea europaea L.) leaf extracts. Food Chemistry 2017.220:282-288.
IF: 4,529
Q: 1 D: 1  

Molina-Calle, M, Priego-Capote, F, Luque de Castro, MD. Characterization of Stevia leaves by LC–QTOF MS/MS analysis of polar and non-polar extracts. Food Chemistry 2017.219:329-338.
IF: 4,529
Q: 1       D: 1  

Peralbo-Molina A, Calderon-Santiago M, Jurado-Gamez B, de Castro MD, Priego-Capote, F. Exhaled breath condensate to discriminate individuals with different smoking habits by GC-TOF/MS. Scientific Reports 2017.7:1421.
IF: 4,259
Q: 1   

Calderon-Santiago M, Lopez-Bascon MA, Peralbo-Molina A, Priego-Capote F. MetaboQC: A tool for correcting untargeted metabolomics data with mass spectrometry detection using quality controls. Talanta 2017.174:29-37.
IF: 4,162
Q: 1   

Molina-Calle M, Sánchez de Medina VS, Priego-Capote F, Luque de Castro MD. Establishing compositional differences between fresh and black garlic by a metabolomics approach based on LC–QTOF MS/MS analysis. Journal of Food Composition and Analysis 2017.62:155-163.
IF: 2,752
Q: 1   

Molina-Calle M, Sánchez de Medina V. Calderón-Santiago M, Priego-Capote F, Luque de Castro MD. Untargeted analysis to monitor metabolic changes of garlic along heat treatment by LC–QTOF MS/MS. Electrophoresis 2017.38:2349-2360.
IF: 2,744
Q: 2   

Molina-Calle M, Priego-Capote F, Luque de Castro MD. Headspace−GC–MS volatile profile of black garlic vs fresh garlic: Evolution along fermentation and behavior under heating. LWT - Food Science and Technology 2017.80:98-105.
IF: 2,329
Q: 1   

Sánchez de Medina V, Miho H, Melliou E, Magiatis P, Priego-Capote F, Luque de Castro MD. Quantitative method for determination of oleocanthal and oleacein in virgin olive oils by liquid chromatography–tandem mass spectrometry. Talanta 2017.162:24-31.
IF: 4,162
Q: 1

Castillo-Peinado LS, Luque de Castro MD. An overview on forensic analysis devoted to analytical chemists. Talanta 2017.167:181-192.
IF: 4,162
Q: 1

Luque-Córdoba D, Luque de Castro MD. Metabolomics: A potential way to know the role of vitamin D on multiple sclerosis. Journal of Pharmaceutical and Biomedical Analysis 2017.136:22-31.
IF: 3,255
Q: 1

Other Publications 2017

Mitsuwan W, Olaya-Abril A, Calderon-Santiago M, Jimenez-Munguia I, Gonzalez-Reyes JA, Priego-Capote F, Voravuthikunchai SP, Rodriguez-Ortega MJ. Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae. Scientific Reports. 2017.7:2715.
IF: 4,259
Q: 1 

Ibanez MI, Cabello P, Luque-Almagro VM, Saez LP, Olaya A, Sánchez de Medina V, Luque de Castro MD, Moreno-Vivian C, Roldan MD. Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). Plos One. 2017.12(3):-e0172908.
IF: 2,806
Q: 1 

Serrano-Rodriguez JM, Gomez-Diez M, Esgueva M, Castejon-Riber C, Mena-Bravo A, Priego-Capote F, Ayala N, Caballero JMS, Munoz A. Pharmacokinetic/pharmacodynamic modeling of benazepril and benazeprilat after administration of intravenous and oral doses of benazepril in healthy horses. Research in Veterinary Science. 2017.114():117-122.
IF: 1,298
Q: 2 

Members

Dra. María Dolores Luque de Castro

Co-Principal Investigator

Team

Feliciano Priego Capote María Dolores Luque de Castro Mónica Calderón Santiago Carlos Augusto Ledesma Escobar Mª del Mar Delgado Povedano Mª Asunción López Bascón Azahara Díaz Lozano Laura de Los Santos Castillo Peinado Diego Luque Córdoba Antonio Mena Bravo Inmaculada Criado Navarro