Research groups

GC21 Metabolomics and identification of bioactive compounds

Our group is mid-size team formed by 9 people. It is a consolidated group that is dedicated to basic transversal research. Our main scientific activity is focused on the development and application of metabolomics methods for application in clinical and agrofood areas.

We are specialized in targeted and untargeted metabolomics analysis by using mass spectrometry. For this purpose, we are well equipped with GC-MS and LC-MS/MS equipment both in high and low resolution acquisition modes to be implemented in metabolomics analysis. In targeted analysis our main objective is to enhance sensitivity and selectivity as well as to obtain a high automation level. In this context, we have contributed to the development of a number of analytical methods with applicability in clinical analysis (vitamin D and metabolites, lipid inflammation markers, phospholipids, etc.) and agrofood characterization (phenolic compounds in olive oil, steviol glycosides, etc.). Concerning untargeted analysis our aim is to combine analytical methods to maximize the number of detected metabolites in a specific sample.

We also develop analytical methods for characterization of biological samples with special emphasis on biofluids and tissues. Finally, a pipeline for identification, configuration of biomarkers panels, and further validation is also available to be applied in metabolomics analysis.

Due to our transversal focus we collaborate with clinical groups to offer a complementary view to be implemented in biomedical studies. Additionally, our group is active in the development of research projects with public and private companies which take benefits from the methodological innovation proposed by our group.

Research Lines


    CIBERFES - Cooperative Research Thematic Network on Aging and Frailty (Collaborator)

    MetaboRed – Red Española de Metabolómica (Collaborator)

    Dr Priego Capote is the coordinator of the FQM-227 - Analytical Chemistry Research Group of the Andalusian Plan for Research, Development and Innovation (PAIDI).  


  • metabolomics
  • mass spectrometry
  • targeted analysis
  • untargeted analysis
  • sample preparation
  • clinical analysis
  • agrofood
  • bioactive compounds



Ongoing projects


Feliciano Priego Capote; Desarrollo de estrategias para la mejora de la detección por espectrometría de masas en análisis metabolómico orientado y global aplicado a clínica y nutrición.; Funding Agency: MINISTERIO DE ASUNTOS ECONÓMICOS Y TRANSFORMACIÓN DIGITAL; Reference: CTQ2015-68813-R

Feliciano Priego Capote; Desarrollo y aplicación de métodos de adquisición independiente de los datos por espectrometría de masas en tándem para su aplicación en análisis metabolómico (DIA-MS2); Funding Agency: AGENCIA ESTATAL DE INVESTIGACIÓN; Reference: PID2019-111373RB-I00


Ongoing projects


Priego Capote, F. Strategy development for improving the mass-spectrometric detection in guided metabiological analysis and global applied to clinical and nutrition. Funding agency: Spanish Ministry of Economy and Competitiveness (MINECO). Reference: CTQ2015-68813-R.

Caracterización de las fracciones funcionales de la aceituna, formulación de nuevos productos y análisis de sus efectos: NUTRADAF. Funding agency: CDTI Feder Interconecta. Reference: ITC-20161265.


Priego Capote, F. Aristoil Project. Funging agency: European Commission, Interreg-Med Program. Reference: MED-1033

Finished projects

Desarrollo de grasas avanzadas GR2020. Funding agency: CDTI Feder Interconecta. Reference: ITC-20151323.


Publications in 2020

Lopez-Bascon MA, Calderon-Santiago M, Diaz-Lozano A, Camargo A, Lopez-Miranda J, Priego-Capote F. Development of a qualitative/quantitative strategy for comprehensive determination of polar lipids by LC-MS/MS in human plasma. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2020. 412 (2):489-498 DOI: 10.1007/s00216-019-02261-8 
IF: 3,637 Q: 1

Luque-Cordoba D, Lopez-Bascon MA, Priego-Capote F. Development of a quantitative method for determination of steroids in human plasma by gas chromatography-negative chemical ionization-tandem mass spectrometry. TALANTA. 2020. 220 ():- DOI: 10.1016/j.talanta.2020.121415 
IF: 5,339 Q: 1

Delgado-Povedano MM, Castillo-Peinado LS, Calderon-Santiago M, de Castro MDL, Priego-Capote F. Dry sweat as sample for metabolomics analysis. TALANTA. 2020. 208 ():- DOI: 10.1016/j.talanta.2019.120428 
IF: 5,339 Q: 1

Criado-Navarro I, Lopez-Bascon MA, Priego-Capote F. Evaluating the Variability in the Phenolic Concentration of Extra Virgin Olive Oil According to the Commission Regulation (EU) 432/2012 Health Claim. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2020. 68 (34):9070-9080 DOI: 10.1021/acs.jafc.0c02380 
IF: 4,192 Q: 1 D: 1

Mayneris-Perxachs J, Arnoriaga-Rodriguez M, Luque-Cordoba D, Priego-Capote F, Perez-Brocal V, Moya A, Burokas A, Maldonado R, Fernandez-Real JM. Gut microbiota steroid sexual dimorphism and its impact on gonadal steroids: influences of obesity and menopausal status. MICROBIOME. 2020. 8 (1):- DOI: 10.1186/s40168-020-00913-x 
IF: 11,607 Q: 1 D: 1

de Castro MDL, Quiles-Zafra R. Lipidomics: An omics discipline with a key role in nutrition. TALANTA. 2020. 219 ():- DOI: 10.1016/j.talanta.2020.121197 
IF: 5,339 Q: 1

Fernandez-Vega A, Chicano-Galvez E, Prentice BM, Anderson D, Priego-Capote F, Lopez-Bascon MA, Calderon-Santiago M, Avendano MS, Guzman-Ruiz R, Tena-Sempere M, Fernandez JA, Caprioli RM, Malagon MM. Optimization of a MALDI-Imaging protocol for studying adipose tissue-associated disorders. TALANTA. 2020. 219 ():- DOI: 10.1016/j.talanta.2020.121184 
IF: 5,339 Q: 1

Criado-Navarro I, Mena-Bravo A, Calderon-Santiago M, Priego-Capote F. Profiling analysis of phospholipid fatty acids in serum as a complement to the comprehensive fatty acids method. JOURNAL OF CHROMATOGRAPHY A. 2020. 1619 ():- DOI: 10.1016/j.chroma.2020.460965 
IF: 4,049 Q: 1

Lope V, del Pozo MD, Criado-Navarro I, Perez-Gomez B, Pastor-Barriuso R, Ruiz E, Castello A, Lucas P, Sierra N, Salas-Trejo D, Llobet R, Martinez I, Romieu I, Chajes V, Priego-Capote F, Pollan M. Serum Phospholipid Fatty Acids and Mammographic Density in Premenopausal Women. JOURNAL OF NUTRITION. 2020. 150 (9):2419-2428 DOI: 10.1093/jn/nxaa168 
IF: 4,281 Q: 1

del Pozo MD, Lope V, Criado-Navarro I, Pastor-Barriuso R, de Larrea NF, Ruiz E, Castello A, Lucas P, Sierra A, Romieu I, Chajes V, Priego-Capote F, Perez-Gomez B, Pollan M. Serum Phospholipid Fatty Acids Levels, Anthropometric Variables and Adiposity in Spanish Premenopausal Women. NUTRIENTS. 2020. 12 (6):- DOI: 10.3390/nu12061895 
IF: 4,546 Q: 1

Lope V, Guerrero-Zotano A, Casas A, Baena-Canada JM, Bermejo B, Perez-Gomez B, Criado-Navarro I, Antolin S, Sanchez-Rovira P, Ramos-Vazquez M, Anton A, Castello A, Garcia-Saenz JA, Munoz M, de Juan A, Andres R, Llombart-Cussac A, Hernando B, Franquesa RM, Caballero R, Priego-Capote F, Martin M, Pollan M. Serum Phospholipids Fatty Acids and Breast Cancer Risk by Pathological Subtype. NUTRIENTS. 2020. 12 (10):- DOI: 10.3390/nu12103132 
IF: 4,546 Q: 1

Miho H, Moral J, Lopez-Gonzalez MA, Diez CM, Priego-Capote F. The phenolic profile of virgin olive oil is influenced by malaxation conditions and determines the oxidative stability. FOOD CHEMISTRY. 2020. 314 ():- DOI: 10.1016/j.foodchem.2020.126183 
IF: 6,306 Q: 1 D: 1

Delgado-Povedano MM, Callado CSC, Priego-Capote E, Ferreiro-Vera C. Untargeted characterization of extracts from Cannabis sativa L. cultivars by gas and liquid chromatography coupled to mass spectrometry in high resolution mode. TALANTA. 2020. 208 ():- DOI: 10.1016/j.talanta.2019.120384 
IF: 5,339 Q: 1

Publications in 2019

Criado-Navarro I, Mena-Bravo A, Calderon-Santiago M, Priego-Capote F; Determination of glycerophospholipids in vegetable edible oils: Proof of concept to discriminate olive oil categories. FOOD CHEMISTRY. 2019 Nov 30;299:125136. doi: 10.1016/j.foodchem.2019.125136.
IF: 5,399 D: 1

Fernandez-Bedmar Z, Anter J, Alonso-Moraga A, de la Torre PD, Luque de Castro MD, Millan-Ruiz Y, Sanchez-Frias M, Guil-Luna S; Red and White Wine Lees Show Inhibitory Effects on Liver Carcinogenesis. MOLECULAR NUTRITION & FOOD RESEARCH. 2019 May;63(9):e1800864. doi: 10.1002/mnfr.201800864. 
IF: 5,151 D: 1

Lopez-Bascon MA, Calderon-Santiago M, Arguello H, Morera L, Garrido JJ, Priego-Capote F; Comprehensive analysis of pig feces metabolome by chromatographic techniques coupled to mass spectrometry in high resolution mode: Influence of sample preparation on the identification coverage. TALANTA. 2019 Jul 1;199:303-309. doi: 10.1016/j.talanta.2019.02.073. 
IF: 4,916 Q: 1

Castillo-Peinado LS, Lopez-Bascon MA, Mena-Bravo A, Luque de Castro MD, Priego-Capote F; Determination of primary fatty acid amides in different biological fluids by LC-MS/MS in MRM mode with synthetic deuterated standards: Influence of biofluid matrix on sample preparation. TALANTA. 2019 Feb 1;193:29-36. doi: 10.1016/j.talanta.2018.09.088.
IF: 4,916 Q: 1

Mena-Bravo A, Calderon-Santiago M, Luque de Castro MD, Priego-Capote F; Evaluation of short-term storage prior to analysis of vitamin D-3 and metabolites in human serum by liquid chromatography coupled to tandem mass spectrometry. TALANTA. 2019 Jun 1;198:344-349. doi: 10.1016/j.talanta.2019.02.030. 
IF: 4,916 Q: 1

Luque de Castro MD, Jurado-Lopez A; The role of analytical chemists in the research on the cultural heritage. TALANTA. 2019 Dec 1;205:120106. doi: 10.1016/j.talanta.2019.07.001.
IF: 4,916

Brito RE, Priego-Capote F, Ledesma-Escobar C, Montoya MR, Mellado JMR; Electrochemical Oxidation Pathways of Hydroxycoumarins on Carbon Electrodes Examined by LSCV and LC-MS/MS. JOURNAL OF THE ELECTROCHEMICAL SOCIETY. 2019. 166(8):H331-H335. doi: 10.1149/2.0781908jes
IF: 3,12

Ledesma-Escobar CA, Priego-Capote F, Robles-Olvera VJ, Garcia-Torres R, De Corcuera JIR, de Castro MDL; GC-MS study of changes in polar/mid-polar and volatile compounds in Persian lime (Citrus latifolia) during fruit growth. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE. 2019 Feb;99(3):1020-1028. doi: 10.1002/jsfa.9266.
IF: 2,422 Q: 1 

Lope V, Toribio MJ, Perez-Gomez B, Castello A, Mena-Bravo A, Sierra MA, Lucas P, Herran-Vidaurrazaga MD, Gonzalez-Vizcayno C, Pino MN, Cruz-Campos I, Roca-Navarro MJ, Aragones N, Romieu I, Martinez-Cortes M, de Castro MDL, Pollan M; Serum 25-hydroxyvitamin D and mammographic density in premenopausal Spanish women. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2019 May;189:101-107. doi: 10.1016/j.jsbmb.2019.03.004.
IF: 3,785 Q: 2

Delgado-Povedano MM, de Castro MDL; The 'in medium virtus' assessment of green analytical chemistry. CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY. 19. 8-14. SI. doi: 10.1016/j.cogsc.2019.02.008. 

Publications in 2018

Miho H, Diez CM, Mena-Bravo A, de Medina VS, Moral J, Melliou E, Magiatis P, Rallo L, Barranco D, Priego-Capote F. Cultivar influence on variability in olive oil phenolic profiles determined through an extensive germplasm survey. FOOD CHEMISTRY. 2018; 266:192-199.
IF: 4,946 Q: 1 D: 1

Ledesma-Escobar CA, Priego-Capote F, Robles-Olvera VJ, Luque de Castro MD. Targeted analysis of the concentration changes of phenolic compounds in Persian lime (Citrus latifolia) during fruit growth. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2018; 66(8):1813-1820.
IF: 3,412 Q: 1 D: 1

Delgado-Povedano MM, Calderon-Santiago M, Luque de Castro MD, Priego-Capote F. Metabolomics analysis of human sweat collected after moderate exercise. TALANTA. 2018; 177:47-65.
IF: 4,244 Q: 1

Lopez-Bascon MA, Calderon-Santiago M, Sanchez-Ceinos J, Fernandez-Vega A, Guzman-Ruiz R, Lopez-Miranda J, Malagon MM, Priego-Capote F. Influence of sample preparation on lipidomics analysis of polar lipids in adipose tissue. TALANTA 2018; 177:86-93.
IF: 4,244 Q: 1

Luque-Cordoba D, Calderon-Santiago M, Luque de Castro MD, Priego-Capote F. Study of sample preparation for determination of endocannabinoids and analogous compounds in human serum by LC-MS/MS in MRM mode. TALANTA 2018; 185:602-610.
IF: 4,244 Q: 1

Luque de Castro MD, Priego-Capote F. The analytical process to search for metabolomics biomarkers. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. 2018; 147:341-349.
IF: 2,831 Q: 2

Tsolakou A, Diamantakos IP, Kalaboki I, Mena-Bravo A, Priego-Capote F, Abdallah IM, Kaddoumi A, Melliou E, Magiatis P. Oleocanthalic Acid, a Chemical Marker of Olive Oil Aging and Exposure to a High Storage Temperature with Potential Neuroprotective Activity. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2018; 66(28):7337-7346.
IF: 3,412 Q: 1 D: 1

Moreno P, Jimenez-Jimenez C, Garrido-Rodriguez M, Calderon-Santiago M, Molina S, Lara-Chica M, Priego-Capote F, Salvatierra A, Munoz E, Calzado MA. Metabolomic profiling of human lung tumor tissues - nucleotide metabolism as a candidate for therapeutic interventions and biomarkers. MOLECULAR ONCOLOGY. 2018; 12(10):1778-1796.
IF: 5,264 Q: 1

Arguello H, Estelle J, Zaldivar-Lopez S, Jimenez-Marin A, Carvajal A, Lopez-Bascon MA, Crispie F, O'Sullivan O, Cotter PD, Priego-Capote F, Morera L, Garrido JJ. Early Salmonella Typhimurium infection in pigs disrupts Microbiome composition and functionality principally at the ileum mucosa. SCIENTIFIC REPORTS. 2018; 8: Núm artículo 7788.
IF: 4,122 Q: 1

Lope V, Castello A, Mena-Bravo A, Amiano P, Aragones N, Fernandez-Villa T, Guevara M, Dierssen-Sotos T, Fernandez-Tardon G, Castano-Vinyals G, Marcos-Gragera R, Moreno V, Salas-Trejo D, Diaz-Santos M, Oribe M, Romieu I, Kogevinas M, Priego-Capote F, Perez-Gomez B, Pollan M. Serum 25-hydroxyvitamin D and breast cancer risk by pathological subtype (MCC-Spain). JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2018; 182:4-13.
IF: 4,095 Q: 1

Rallo L, Diez CM, Morales-Sillero A, Miho H, Priego-Capote F, Rallo P. Quality of olives: A focus on agricultural preharvest factors. SCIENTIA HORTICULTURAE. 2018; 233:491-509.
IF: 1,76 Q: 1

Garcia-Valverde MT, Ledesma-Escobar CA, Lucena R, Cardenas S. Tunable Polarity Carbon Fibers, a Holistic Approach to Environmental Protection. MOLECULES. 2018; 23(5): Núm artículo 1026.
IF: 3,098 Q: 2

Jimenez-Munguia I, Calderon-Santiago M, Rodriguez-Franco A, Priego-Capote F, Rodriguez-Ortega MJ. Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4. PEERJ. 2018; 6: Núm artículo e4966.
IF: 2,118 Q: 2

Publications in 2017

Ledesma-Escobar C.A, Priego-Capote F, Robles-Olvera VJ, Luque de Castro MD. Changes in the composition of the polar fraction of Persian lime (Citrus latifolia) during fruit growth by LC–QTOF MS/MS analysis. Food Chemistry 2017.234:262-268.
IF: 4,529 Q: 1 D: 1

Delgado-Povedano MD, Priego-Capote F, de Castro M.D. Selective ultrasound-enhanced enzymatic hydrolysis of oleuropein to its aglycon in olive (Olea europaea L.) leaf extracts. Food Chemistry 2017.220:282-288.
IF: 4,529 Q: 1 D: 1  

Molina-Calle, M, Priego-Capote, F, Luque de Castro, MD. Characterization of Stevia leaves by LC–QTOF MS/MS analysis of polar and non-polar extracts. Food Chemistry 2017.219:329-338.
IF: 4,529 Q: 1 D: 1  

Peralbo-Molina A, Calderon-Santiago M, Jurado-Gamez B, de Castro MD, Priego-Capote, F. Exhaled breath condensate to discriminate individuals with different smoking habits by GC-TOF/MS. Scientific Reports 2017.7:1421.
IF: 4,259 Q: 1   

Calderon-Santiago M, Lopez-Bascon MA, Peralbo-Molina A, Priego-Capote F. MetaboQC: A tool for correcting untargeted metabolomics data with mass spectrometry detection using quality controls. Talanta 2017.174:29-37.
IF: 4,162 Q: 1   

Molina-Calle M, Sánchez de Medina VS, Priego-Capote F, Luque de Castro MD. Establishing compositional differences between fresh and black garlic by a metabolomics approach based on LC–QTOF MS/MS analysis. Journal of Food Composition and Analysis 2017.62:155-163.
IF: 2,752 Q: 1   

Molina-Calle M, Sánchez de Medina V. Calderón-Santiago M, Priego-Capote F, Luque de Castro MD. Untargeted analysis to monitor metabolic changes of garlic along heat treatment by LC–QTOF MS/MS. Electrophoresis 2017.38:2349-2360.
IF: 2,744 Q: 2   

Molina-Calle M, Priego-Capote F, Luque de Castro MD. Headspace−GC–MS volatile profile of black garlic vs fresh garlic: Evolution along fermentation and behavior under heating. LWT - Food Science and Technology 2017.80:98-105.
IF: 2,329 Q: 1   

Sánchez de Medina V, Miho H, Melliou E, Magiatis P, Priego-Capote F, Luque de Castro MD. Quantitative method for determination of oleocanthal and oleacein in virgin olive oils by liquid chromatography–tandem mass spectrometry. Talanta 2017.162:24-31.
IF: 4,162 Q: 1

Castillo-Peinado LS, Luque de Castro MD. An overview on forensic analysis devoted to analytical chemists. Talanta 2017.167:181-192.
IF: 4,162 Q: 1

Luque-Córdoba D, Luque de Castro MD. Metabolomics: A potential way to know the role of vitamin D on multiple sclerosis. Journal of Pharmaceutical and Biomedical Analysis 2017.136:22-31.
IF: 3,255 Q: 1

Mitsuwan W, Olaya-Abril A, Calderon-Santiago M, Jimenez-Munguia I, Gonzalez-Reyes JA, Priego-Capote F, Voravuthikunchai SP, Rodriguez-Ortega MJ. Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae. Scientific Reports. 2017.7:2715.
IF: 4,259 Q: 1 

Ibanez MI, Cabello P, Luque-Almagro VM, Saez LP, Olaya A, Sánchez de Medina V, Luque de Castro MD, Moreno-Vivian C, Roldan MD. Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). Plos One. 2017.12(3):-e0172908.
IF: 2,806 Q: 1 

Serrano-Rodriguez JM, Gomez-Diez M, Esgueva M, Castejon-Riber C, Mena-Bravo A, Priego-Capote F, Ayala N, Caballero JMS, Munoz A. Pharmacokinetic/pharmacodynamic modeling of benazepril and benazeprilat after administration of intravenous and oral doses of benazepril in healthy horses. Research in Veterinary Science. 2017.114():117-122.
IF: 1,298 Q: 2 


Principal Investigator
Feliciano Priego Capote

Co-Principal Investigator
María Dolores Luque De Castro

Post-Doctoral Researchers
Mónica Calderón Santiago
Carlos Augusto Ledesma Escobar
María Asunción López Bascón

Pre-Doctoral Researchers
Laura De Los Santos Castillo Peinado
Inmaculada Criado Navarro
Diego Luque Córdoba
Sonia Tomé Rodríguez