Research groups

GC24 Clinical and Molecular Microbiology

The Consolidated Research Group of Clinical and Molecular Microbiology integrates (1) Clinical Microbiology Staff and Residents of the Unit of Microbiology, University Hospital Reina Sofía, (2) Professors of the Department of Microbiology-Medical School, University of Cordoba, and (3) Other Researchers and Technicians with a contractual tie to our group.

The research interests of the group include:

  • Antimicrobial resistance in bacteria of medical interest, with special attention to multiresistant gram-negative organisms.
  • In vitro activity of new antimicrobial agents.
  • Diagnostic methods in Clinical Microbiology.

In the area of major interest for the group (antimicrobial resistance), we consider both Enterobacteria (Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., etc.) and non-fermenters (Pseudomonas aeruginosa and other species, Actinetobacter baumannii and related species, Stenotrophomonas   maltophilia, among others). Other studies are focused on methicillin-resistant Staphylococcus aureus and coryneform bacteria.

Our objectives include: (1) To study mechanisms causing low-level resistance: porin alterations, (over)production of efflux pumps, (2) To study mechanisms of resistance to specific antimicrobial groups, extended-sepctrum beta-lactamases, carbapenemases, plasmid-mediated quinolone resistance, type II topoisomerases, aminoglycoside-modifying enzymes, ARN 16S methyl-transferases)…, and (3) To characterize genes and mobile elements (plasmids, transposons, integrons, gene cassettes, etc.) involved in antimicrobial resistance.

We are also considering molecular epidemiology of multiresistant bacteria causing nosocomial infections, and the study of the relationship between antimicrobial resistance and virulence.

The achievements of our research include:

  • Discovery of qnr genes, causing plasmid-mediated quinolone resistance. Detailed contribution of these genes to resistance and design of molecular methods for their detection.
  • Demonstration of the importance of porins in of low-level resistance.
  • Analysis of penicillin-binding proteins of multiresistant A. baumannii.
  • Characterization of mechanisms of resistance to aminoglycosides in multiresistant enterobacteria, non-fermenters and corynebacteria.
  • Demonstration of the importance of manganese contents in culture media when testing tigecycline.
  • Collaboration in multiples clinical studies on treatment and prognosis of infections caused by multiresistant bacteria.

We contribute to the Programs “Resistance”  and “Infections in Transplanted Patients” of the Spanish network for research on infectious diseases (REIPI, http://reipi.org/)  supported by the  Institute of Health Carlos III  (ISCIII). This network has developed and is developing different multicenter studies on clinical and microbiological aspects of infections caused by resistant bacteria of clinical relevance.

Research Lines

  • Beta-lactamases: Identification of genes coding for extended-espectrum beta-lactamases, AmpC-type beta-lactamases, carbapenemases and other beta-lactamases of clinical importance.
  • Plasmid-mediated quinolone resistance: Qnr proteins and related mechanisms.
  • Mechanisms of aminoglycoside resistance: Modifying enzymes and ARN 16S methyl-transferases.
  • Low level resistance mechanisms, with interest in outer membrane proteins (particularly porins in enterobacterias and in Pseudomonas spp.) and efflux pumps.
  • Heteroresistance to antimicrobial agents, focused on carbapenem and polymyxins.
  • Genetic elements involved in horizontal gene transfer and its relevance for antimicrobial resistance.
  • Bacterial targets: Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., other enterobacterias, Pseudomonas aeruginosa (and related species), Acinetobacter baumannii complex, Stenotrophomonas maltophilia, Staphylococcus aureus, coagulase-negative staphylococci, Corynebacterium and related genera.
  • Development of new antimicrobial agents: chelocardin, new aminoglycosides.
  • In vitro studies of new compounds, in collaboration with the pharmaceutical industry.
  • Evaluation of Maldi-Tof for identification of microorgansims of clinical importance, including both bacteria and fungi.
  • Use of molecular methods for identification of clinically important microorganisms.
  • Evaluation of new commercial methods and assays for microbiological diagnosis.
  • Evaluation of commercial and in-house methods for susceptibility testing of bacteria of clinical interest.

Networks

    REIPI - Spanish Network for Research on Infectious Diseases 

    Andalusian Network For Clinical Research In Infectious Diseases. RIC-0537-2018.

Keywords

  • antimicrobial-resistance
  • beta-lactamases
  • aminoglycoside-modifying-enzymes
  • methyl-transferases
  • Qnr
  • quinolones
  • polymyxins
  • carbapenems
  • cephalosporins
  • heteroresistance
  • antibiogram
  • susceptibility-testing
  • molecular-typing
  • enterobacteria
  • pseudomonas
  • acinetobacter

Additional Information

Ongoing Projects

Heteroresistance and persistance in carbapenem-resistant Klebsiella pneumoniae. PI: Luis Martínez-Martínez. Funding agency: National Institute of Health Carlos III (ISCIII). Reference PI14/01911. 2015-2019.

Study of epigenetic modifications induced by cytomegalovirus as risk and prognostic markers. PI: Irene Gracia-Ahufinger. Funding agency: National Institute of Health Carlos III (ISCIII). Reference: PI14/01225. 2015-2019.

Importance of the selection of stable mutants and persisters on colistin heteroresistance in Klebsiella pneumoniae. PI: Luis Martínez-Martínez. Funding agency: National Institute of Health Carlos III (ISCIII) Reference PI17-02241. 2018-2020.

Spanish National Survey on Pseudomonas aeruginosa Antimicrobial Resistance and Epidemiology: Detecting High-risk Clones to Optimize Antipseudomonal Treatments. PI: Antonio Oliver (Mallorca). Co-PI: Luis Martínez-Martínez. Funding agency: Merck, Sharp & Dhome. 2017-2018.

Precision diagnosis and treatment in Infectious Diseases and Cancer. Challenge: Analysis of microbial susceptibility and resistance to antibiotics (ASURANT). IPs Asurant: José Miguel Cisneros and Luis Martínez Martínez. Funding Agency: Ministry of Economy and Industry and Andalusian Health System. 2018-2022.

Study of the mechanisms of resistance to cetazidime-avibactam in isolates of Klebsiella pneumoniae producing KPC-3 cultured form patients under ceftazidime-avibactam therapy. Funding agency: Pfizer GEP, SLU. 2018-2019.

Epidemiological study to estimate the distribution of O serotypes among extraintestinal pathogenic Escherichia coli (ExPEC) from hospitalized patients of any age group with invasive disease by ExpEC ant to evaluate the definition of clinical case and risk factors. Funding Agency: Janssen-Cilag International NV. 2019-2020.

Recent Publications (2017-2019)

2017

Sánchez-Diener I, et al. Interplay among resistance profiles, high-risk clones and virulence in the Caenorhabditis elegans Pseudomonas aeruginosa infection model. Antimicrob Agents Chemother. 2017, 61: pii: AAC.01586-17.

Del Barrio-Tofiño E, et al. Genomics and Susceptibility Profiles of Extensively Drug-Resistant Pseudomonas aeruginosa Isolates from Spain. Antimicrob Agents Chemother. 2017: 61. pii: e01589-17.

Alibi S, et al. Occurrence of Corynebacterium striatum as an emerging antibiotic-resistant nosocomial pathogen in a Tunisian hospital. Sci Rep. 2017 Aug 28;7:9704.

Lázaro-Díez M, et al. Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii. Sci Rep. 2017 Jul 4;7:4571.

Machuca I, et al. Mortality Associated with  Bacteremia Due to Colistin-Resistant Klebsiella pneumoniae with High-Level Meropenem Resistance: Importance of Combination Therapy without Colistin and Carbapenems. Antimicrob Agents Chemother 2017 Jul 25;61. pii: e00406-17.

Delgado-Valverde M et al. MIC of amoxicillin/clavulanate according to CLSI and EUCAST: discrepancies and clinical impact in patients with bloodstream infections due to Enterobacteriaceae. J Antimicrob Chemother 2017 May 1;72:1478-1487.

Gonzalez-Garcia FJ, et al. Fatal bilateral haemopneumothoraces following double-lung transplantation caused by pretransplant colonization by Scedosporium apiospermum. Eur J Cardiothorac Surg. 2017 Jun 1;51:1209.

Ruiz P, Causse M, Vaquero M, Gutierrez JB, Casal M. Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis. Infecti Drug Resist 2017, 10:463-467.

2018

Quiñonero A, del Molino ICP, de la Fuente CG, Sanjuan MC, Aguero J, Martinez-Martinez L. Phenotypic detection of clinical isolates of Haemophilus influenzae with altered penicillin-binding protein 3. Eur J Clin Microbiol Infect Dis 2018; 37:1475-1480.

Fernandez-Martinez M, del Castillo BR, Lecea-Cuello MJ, Rodriguez-Bano J, Pascual A, Martinez-Martinez L. Prevalence of Aminoglycoside-Modifying Enzymes in Escherichia coli and Klebsiella pneumoniae Producing Extended Spectrum B-Lactamases Collected in Two Multicenter Studies in Spain. Microb Drug Resist 2018; 24:367-376.

Gutierrez-Aroca JB, Ruiz P, Vaquero M, Causse M, Casal M. Surveillance of Drug-Resistant Tuberculosis in Spain (2001-2015). Microb Drug Resist 2018; 24:839-843.

Gutierrez-Aroca JB, Ruiz-Martinez P, Rodriguez-Ruiz L, Casal-Roman M. Distribution of hepatitis C genotypes in the city of Cordoba. Rev Esp Quimioter 2018; 31:164-165.

Cano A, Gutierrez-Gutierrez B, Machuca I, Gracia-Ahufinger I, Perez-Nadales E, Causse M, Caston JJ, Guzman-Puche J, Torre-Gimenez J, Kindelan L, Martinez-Martinez L, Rodriguez-Bano J, Torre-Cisneros J. Risks of Infection and Mortality Among Patients Colonized With Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae: Validation of Scores and Proposal for Management. Clin Infect Dis 2018: 66:1204-1210.

Amat T, Gutierrez-Pizarraya A, Machuca I, Gracia-Ahufinger I, Perez-Nadales E, Torre-Gimenez A, Garnacho-Montero J, Cisneros JM, Torre-Cisneros J. The combined use of tigecycline with high-dose colistin might not be associated with higher survival in critically ill patients with bacteraemia due to carbapenem-resistant Acinetobacter baumannii. Clin Microbiol Infect 2018: 24:630-634.

Espinel-Ingroff A, Turnidge J, Alastruey-Izquierdo A, Dannaoui E, Garcia-Effron G, Guinea J, Kidd S, Pelaez T, Sanguinetti M, Meletiadis J, Botterel F, Bustamante B, Chen YC, Chakrabarti A, Chowdhary A, Chryssanthou E, Cordoba S, Gonzalez GM, Guarro J, Johnson EM, Kus JV, Lass-Florl C, Linares-Sicilia MJ, Martin-Mazuelos E, Negri CE, Pfaller MA, Tortorano AM. Posaconazole MIC Distributions for Aspergillus fumigatus Species Complex by Four Methods: Impact of cyp51A Mutations on Estimation of Epidemiological Cutoff Values. Antimicrob Agents Chemother 2018: 62: e01916-17.

Espinel-Ingroff A, Turnidge J, Alastruey-Izquierdo A, Botterel F, Canton E, astro C, Chen YC, Chen Y, Chryssanthou E, Dannaoui E, Garcia-Effron G, Gonzalez  GM, Govender NP, Guinea J, Kidd S, Lackner M, Lass-Flörl C, Linares-Sicilia MJ, López-Soria L, Magobo R, Pelaez T, Quindós G, Rodriguez-Iglesia MA, Ruiz MA, Sánchez-Reus F, Sanguinetti M, Shields R, Szweda P, Tortorano A, Wengenack NL, Bramati S, Cavanna C, DeLuca C, Gelmi M, Grancini A, Lombardi G, Meletiadis J, Negri CE, Passera M, Peman J, Prigitano A, Sala E, Tejada M. Method-dependent epidemiological cutoff values (ECVs) for detection of triazole resistance in Candida and Aspergillus species for the SYO colorimetric broth and Etest agar diffusion methods. Antimicrob Agents Chemother 2019, 63: pii: AAC.01651-18.

Alastruey-Izquierdo A, Alcazar-Fuoli L, Rivero-Menendez O, Ayats J, Castro C, Garcia-Rodriguez J, Goterris-Bonet L, Ibanez-Martinez E, Linares-Sicilia MJ, Martin-Gomez MT, Martin-Mazuelos E, Pelaez T, Peman J, Rezusta A, Rojo S, Tejero R, Anza DV, Vinuelas J, Zapico MS, Cuenca-Estrella M. Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study). Antimicrob Agents Chemother 2018: 62: e00358-18.

Hamzaoui Z, Ocampo-Sosa A, Martinez MF, Landolsi S, Ferjani S, Maamar E, Saidani M, Slim A, Martinez-Martinez L, Boutiba-Ben Boubaker I. Role of association of OmpK35 and OmpK36 alteration and bla(ESBL) and/or bla(AmpC) genes in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae. Int J Antimicrob Agents 2018, 52:898-905.

Garcia-Castillo M, Garcia-Fernandez S, Gomez-Gil R, Pitart C, Oviano M, Gracia-Ahufinger I, Diaz-Reganon J, Tato M, Canton R. Activity of ceftazidime-avibactam against carbapenemase-producing Enterobacteriaceae from urine specimens obtained during the infection-carbapenem resistance evaluation surveillance trial (iCREST) in Spain. Int J Antimicrob Agents 2018, 51:511-515.

Lopez M, Rueda A, Florido JP, Blasco L, Fernandez-Garcia L, Trastoy R, Fernandez-Cuenca F, Martinez-Martinez L, Vila J, Pascual A, Bou G, Tomas M. Evolution of the Quorum network and the mobilome (plasmids and bacteriophages) in clinical strains of Acinetobacter baumannii during a decade. Sci Rep 2018, 8:2523.

Zboromyrska Y, Bosch J, Aramburu J, Cuadros J, Garcia-Riestra C, Guzman-Puche J, Martos CL, Loza E, Munoz-Algarra M, de Alegria CR, Sanchez-Hellin V, Vila J. A multicentre study investigating parameters which influence direct bacterial identification from urine. Plos One. 2018: 13: Núm artículo e0207822.

Fernandez-Garcia L, Ambroa A, Blasco L, Bleriot I, Lopez M, Alvarez-Marin R, Fernandez-Cuenca F, Martinez-Martinez L, Vila J, Rodriguez-Bano J, Gamacho-Montero J, Cisneros JM, Pascual A, Pachon J, Bou G, Smani Y, Tomas M. Relationship Between the Quorum Network (Sensing/Quenching) and Clinical Features of Pneumonia and Bacteraemia Caused by A. baumannii. Front Microbiol 2018; 9: 3105

Cerezales M, Ocampo-Sosa AA, Montes LA, Rios CD, Bustamante Z, Santos J, Martinez-Martinez L, Higgins PG, Gallego L. High Prevalence of Extensively Drug-resistant Acinetobacter baumannii at a Children Hospital in Bolivia. Ped Infect Dis J 2018, 37:1118-1123.

Aguado JM, Silva JT, Fernandez-Ruiz M, Cordero E, Fortun J, Gudiol C, Martinez-Martinez L, Vidal E, Almenar L, Almirante B, Canton R, Carratala J, Caston JJ, Cercenado E, Cervera C, Cisneros JM, Crespo-Leiro MG, Cuervas-Mons V, Elizalde-Fernandez J, Farinas MC, Gavalda J, Goyanes MJ, Gutierrez-Gutierrez B, Hernandez D, Len O, Lopez-Andujar R, Lopez-Medrano F, Martin-Davila P, Montejo M, Moreno A, Oliver A, Pascual A, Perez-Nadales E, Roman-Broto A, San-Juan R, Seron D, Sole-Jover A, Valerio M, Munoz P, Torre-Cisneros J. Management of multidrug resistant Gram-negative bacilli infections in solid organ transplant recipients: SET/GESITRA-SEIMC/REIPI recommendations. Transplant Rev 2018, 32:36-57.

Cercenado E, Canton R, Ramirez MG, Mansilla CA, Ahufinger IG, Ramirez MG, Alarcon JM. Procedures in Clinical Microbiology (number 15.(a), 2nd edition 2018). Enf Infec Microbiol Clin 2018, 36:603-603.

Rodriguez-Lozano J, de Malet A, Cano ME, de la Rubia L, Wallmann R, Martinez-Martinez L, Calvo J. Antimicrobial susceptibility of microorganisms that cause urinary tract infections in pediatric patients. Enf Infec Microbiol Clin 2018, 36:417-422.

2019

Ruiz P, Causse M, Vaquero M, Casal M. In Vitro Activity of Tedizolid against ycobacterium tuberculosis. Antimicrob Agents Chemother. 2019, 63. pii:e01939-18. doi: 10.1128/AAC.01939-18.

Guzmán-Puche J, Gracia-Ahufinger I, Causse M, Tejero-García R, Rodríguez-López FC, Casal-Román M. Combination of Coral UTI Screen(TM) system, gram-stain and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for diagnosis of urinary tract infections directly from urine samples. J Chemother. 2019, 2:74-80.

Kieffer N, Guzmán-Puche J, Poirel L, Kang HJ, Jeon CO, Nordmann P. ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans. J Antimicrob Chemother. 2019, 74:1568-1571.

Castro C, Galán-Sanchez F, Linares MJ, Tejero R, Ruiz M, Serrano ML, Rodríguez-Iglesias M, Martín-Mazuelos E; FUNGAE-IFI Study Group. A prospective survey of Aspergillus spp. in respiratory tract samples: Species identification and susceptibility patterns. Med Mycol. 2019, 57:412-420.

López-Camacho E, Paño-Pardo JR, Sotillo A, Elías-López C, Martínez-Martínez L, Gómez-Gil R, Mingorance J. Meropenem heteroresistance in clinical isolates of OXA-48-producing Klebsiella pneumoniae. Diagn Microbiol Infect Dis 2019, 93:162-166.

Haeili M, Kafshdouz M, Pishnian Z, Feizabadi MM, Martínez-Martínez L. Comparison of susceptibility testing methods for determining the activity of colistin against Gram-negative bacilli of clinical origin. J Med Microbiol 2019, 68:60-66.

Ishikawa M, García-Mateo N, Čusak A, López-Hernández I, Fernández-Martínez M, Müller M, Rüttiger L, Singer W, Löwenheim H, Kosec G, Fujs Š, Martínez-Martínez L, Schimmang T, Petković H, Knipper M, Durán-Alonso MB. Lower ototoxicity and absence of hidden hearing loss point to gentamicin C1a and apramycin as promising antibiotics for clinical use. Sci Rep. 2019, 9:2410.

[Ruiz]de Alegría Puig C[R], Torres MF, Marfil-Pérez E, Ferández MIR, Del Río MC,Balbín JA, Martínez-Martínez L. Comparison between Vitek MS, Bruker Biotyper,Vitek2, and API20E for differentiation of species of the genus Raoultella. Eur J Clin Microbiol Infect Dis. 2019 , 38:467-470.

Cirit OS, Fernández-Martínez M, Yayla B, Martínez-Martínez L. Aminoglycoside resistance determinants in multiresistant Escherichia coli and Klebsiella pneumoniae clinical isolates from Turkish and Syrian patients. Acta Microbiol Immunol Hung. 2019 Feb 26:1-9.

Pérez-Vazquez M, Oteo-Iglesias J, Sola-Campoy PJ, Carrizo-Manzoni H, Bautista V, Lara N, Aracil B, Alhambra A, Martínez-Martínez L, Campos J; Spanish Antibiotic Resistance Surveillance Program collaborating Group. Characterization of carbapenemase-producing Klebsiella oxytoca in Spain, 2016-2017. Antimicrob Agents Chemother 2019 e02529-18.

Palacios-Baena ZR, Delgado-Valverde M, Valiente Méndez A, Almirante B, Gómez-Zorrilla S, Borrell N, Corzo JE, Gurguí M, de la Calle C, García-Álvarez L,Ramos L, Gozalo M, Morosini MI, Molina J, Causse M, Pascual Á, Rodríguez-Baño J; REIPI/GEIRAS-SEIMC BACTERIEMIA-MIC group. Impact of de-escalation on prognosis of patients with bacteraemia due to Enterobacteriaceae: a post-hoc analysis from a multicenter prospective cohort. Clin Infect Dis. 2019, 69: 956-962.

Del Barrio-Tofiño E, Zamorano L, Cortes-Lara S, López-Causapé C, Sánchez-Diener I, Cabot G, Bou G, Martínez-Martínez L, Oliver A;GEMARA-SEIMC/REIPI Pseudomonas study Group . Spanish nationwide survey on Pseudomonas aeruginosa antimicrobial resistance mechanisms and epidemiology. J Antimicrob Chemother. 2019 Apr 15. pii: dkz147.

Ramos-Vivas J, Chapartegui-González I, Fernández-Martínez M, González-Rico C, Fortún J, Escudero R, Marco F, Linares L, Montejo M, Aranzamendi M, Muñoz P, Valerio M, Aguado JM, Resino E, Ahufinger IG, Vega AP, Martínez L, Fariñas MC; ENTHERE Study Group, the Group for Study of Infection in Transplantation of the Spanish Society of Infectious Diseases and Clinical Microbiology (GESITRA-SEIMC) and the Spanish Network for Research in Infectious Diseases (REIPI). Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients. Sci Rep. 2019, 9:8928.

Rodriguez-Gómez J, Pérez-Nadales E, Gutiérrez-Gutiérrez B, Machuca I, Martinez-Martinez L, Rivera F, Cano A, Castón JJ, Robles JC, la Fuente C, Rodríguez-López F, Rodriguez-Baño J, Torre-Cisneros J; Spanish Network for Research in Infectious Diseases (REIPI), Instituto de Salud Carlos III, Madrid, Spain. Prognosis of urinary tract infection caused by KPC-producing Klebsiella pneumoniae: the impact of inappropriate empirical treatment. J Infect 2019,  79: 245-252.

Camprubí-Font C, Ruiz del Castillo B, Barrabés S, Martínez-Martínez L, Martinez-Medina M. Amino acid substitutions and differential gene expression of outer membrane proteins in adherent-invasive Escherichia coli. Front Microbiol 2019, 10: 1707.

Del Barrio-Tofiño E, Sánchez-Diener I, Zamorano L, Cortes-Lara S, López-Causapé C, Cabot G, Bou G, Martínez-Martínez L, Oliver A; GEMARA-SEIMC/REIPI. Association between Pseudomonas aeruginosa O-antigen serotypes, resistance profiles and high-risk clones: results from a Spanish nationwide survey. J Antimicrob Chemother 2019, 74: 3217–3220.

Martínez-Martínez L. Carbapenemases: The never-ending story. Enferm. Infecc. Microbiol. Clin. 2019, 37:73-75.

Paez-Vega A, Cantisan S, Vaquero JM, Vidal E, Luque-Pineda A, Lobo-Acosta MÁ, Pérez AB, Alonso-Moralejo R, Iturbe D, Monforte V, Otero-Gonzalez I, Pastor A, Ussetti P, Torre-Cisneros J. Efficacy and safety of the combination of reduced duration prophylaxis followed by immuno-guided prophylaxis to prevent cytomegalovirus disease in lung transplant recipients (CYTOCOR STUDY): an open-label, randomised, non-inferiority clinical trial. BMJ Open. 2019 Aug 15;9(8):e030648. doi: 10.1136/bmjopen-2019-030648.

Pérez AB, Chueca N, Macías J, Pineda JA, Salmerón J, Rivero-Juárez A, Hidalgo-Tenorio C, Espinosa MD, Téllez F, Von-Wichmann MÁ, Omar M, Santos J, Hernández-Quero J, Antón JJ, Collado A, Lozano AB, García-Deltoro M, Casado M, Pascasio JM, Selfa A, Rosales JM, De la Iglesia A, Arenas JI, García-Bujalance S, Ríos MJ, Bernal E, Martínez O, García-Herola A, Vélez M, Rincón P, García F. Prevalence of resistance associated substitutions and efficacy of baseline resistance-guided chronic hepatitis C treatment in Spain from the GEHEP-004 cohort. PLoS One. 2019 Aug 30;14(8):e0221231. doi: 10.1371/journal.pone.0221231.

Álvarez-Marín R, Navarro-Amuedo D, Gasch-Blasi O, Rodríguez-Martínez JM, Calvo-Montes J, Lara-Contreras R, Lepe-Jiménez JA, Tubau-Quintano F, Cano-García ME, Rodríguez-López F, Rodríguez-Baño J, Pujol-Rojo M, Torre-Cisneros J, Martínez-Martínez L, Pascual-Hernández Á, Jiménez-Mejías ME. A prospective, multicenter case control study of risk factors for acquisition and mortality in Enterobacter species bacteremia. J Infect. 2020 Feb;80(2):174-181. doi: 10.1016/j.jinf.2019.09.017.

Lucia Blasco, Anton Ambroa , Maria Lopez , Laura Fernandez-Garcia , Ines Bleriot , Rocio Trastoy , Jose Ramos-Vivas , Tom Coenye , Felipe Fernandez-Cuenca , Jordi Vila, Luis Martinez-Martinez , Jesus Rodriguez-Baño, Alvaro Pascual , Jose Miguel Cisneros, Jeronimo Pachon, German Bou and Maria Tomas. Combined Use of the Ab105-2ϕ∆CI Lytic Mutant Phage and Different Antibiotics in Clinical Isolates of Multi-Resistant Acinetobacter baumannii. Microorganisms 2019, 7, 556; doi:10.3390/microorganisms7110556

Vrancken B, Cuypers L, Pérez AB, Chueca N, Anton-Basantas J, de la Iglesia A, Fuentes J, Pineda JA, Téllez F, Bernal E, Rincón P, Von Wichman MA, Fuentes A, Vera F, Rivero-Juárez A, Jiménez M, Vandamme AM, García F. Cross-country migration linked to people who inject drugs challenges the long-term impact of national HCV elimination programmes. J Hepatol. 2019 Dec;71(6):1270-1272.

Projects

PROJECTS IN 2020

Ongoing projects

Regional

Ana Belén Pérez Jiménez; Aplicación de la secuenciación masiva para el diagnóstico de infecciones neurológicas de origen vírico no filiadas; Funding Agency: CONSEJERÍA DE SALUD Y FAMILIAS; Reference: 116190130216

National

Luis Martínez Martínez; Importancia de la selección de mutantes estables y de variantes persistentes en la expresión de heterorresistencia a colistina en Klebsiella pneumoniae; Funding Agency: INSTITUTO DE SALUD CARLOS III; Reference: PI17/02241

PROJECTS IN 2019

Ongoing projects

National

Martínez-Martínez, L. Heteroresistance and persistance in carbapenem-resistant Klebsiella pneumoniae. Funding agency: National Institute of Health Carlos III (ISCIII). Reference: PI14/01911. 2015-2019.

Gracia-Ahufinger, I. Study of epigenetic modifications induced by cytomegalovirus as risk and prognostic markers. Funding agency: National Institute of Health Carlos III (ISCIII). Reference: PI14/01225. 2015-2019.

Martínez-Martínez, L. Importance of the selection of stable mutants and persisters on colistin heteroresistance in Klebsiella pneumoniae. Funding agency: National Institute of Health Carlos III (ISCIII). Reference: PI17-02241. 2018-2020.

Martínez-Martínez, L. Increment-SOT study. Funding Agency: Sociedad Andaluza de Transplantes de Órganos y Tejidos. Reference: SATOT_2016_001.

Fernández García, MD. Associated to Miguel Servet Contract. Funding agency: Institute Carlos III Health (ISCIII). Reference: CP18/00067. Associated to Miguel Servet Contract.

Publications

Publications in 2020

Alvarez-Marin R, Navarro-Amuedo D, Gasch-Blasi O, Rodriguez-Martinez JM, Calvo-Montes J, Lara-Contreras R, Lepe-Jimenez JA, Tubau-Quintano F, Cano-Garcia ME, Rodriguez-Lopez F, Rodriguez-Bano J, Pujol-Rojo M, Torre-Cisneros J, Martinez-Martinez L, Pascual. A prospective, multicenter case control study of risk factors for acquisition and mortality in Enterobacter species bacteremia. JOURNAL OF INFECTION. 2020. 80 (2):174-181 DOI: 10.1016/j.jinf.2019.09.017 
IF: 4,842 Q: 1

Fraile-Ribot PA, Zamorano L, Orellana R, del Barrio-Tofino E, Sanchez-Diener I, Cortes-Lara S, Lopez-Causape C, Cabot G, Bou G, Martinez-Martinez L, Oliver A. (GEMARA-SEIMC/REIPI Pseudomonas Study Group: Galan, F; Gracia, I; Rodriguez, MA; Martin, L; Sanc. Activity of Imipenem-Relebactam against a Large Collection of Pseudomonas aeruginosa Clinical Isolates and lsogenic beta-Lactam-Resistant Mutants. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2020. 64 (2):- DOI: 10.1128/AAC.02165-19 
IF: 4,904 Q: 1

Ramos-Vivas J, Chapartegui-Gonzalez I, Fernandez-Martinez M, Gonzalez-Rico C, Barrett J, Fortun J, Escudero R, Marco F, Linares L, Nieto J, Aranzamendi M, Munoz P, Valerio M, Aguado JM, Chaves F, Gracia-Ahufinger I, Paez-Vega A, Martinez-Martinez L, Farinas MC. Adherence to Human Colon Cells by Multidrug ResistantEnterobacteralesStrains Isolated From Solid Organ Transplant Recipients With a Focus onCitrobacter freundii. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY. 2020. 10 ():- DOI: 10.3389/fcimb.2020.00447 
IF: 4,123 Q: 2

Martinez-Medina M, Strozzi F, Del Castillo BR, Serrano-Morillas N, Bustins NF, Martinez-Martinez L. Antimicrobial Resistance Profiles of Adherent Invasive Escherichia coli Show Increased Resistance to beta-Lactams. ANTIBIOTICS-BASEL. 2020. 9 (5):- DOI: 10.3390/antibiotics9050251 
IF: 3,893 Q: 1

Chapartegui-Gonzalez I, Fernandez-Martinez M, Rodriguez-Fernandez A, Rocha DJP, Aguiar ERGR, Pacheco LGC, Ramos-Vivas J, Calvo J, Martinez-Martinez L, Navas J. Antimicrobial Susceptibility and Characterization of Resistance Mechanisms ofCorynebacteriumurealyticumClinical Isolates. ANTIBIOTICS-BASEL. 2020. 9 (7):- DOI: 10.3390/antibiotics9070404 
IF: 3,893 Q: 1

Vaquero-Alvarez E, Cubero-Atienza A, Ruiz-Martinez P, Vaquero-Abellan M, Mecias MDR, Aparicio-Martinez P. Bibliometric Study of Technology and Occupational Health in Healthcare Sector: A Worldwide Trend to the Future. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH. 2020. 17 (18):- DOI: 10.3390/ijerph17186732 
IF: 2,849 Q: 1

Castan JJ, Gallo M, Garcia M, Cano A, Escribano A, Machuca I, Gracia-Aufinger I, Guzman-Puche J, Perez-Nadales E, Recio M, Munoz M, Martinez-Martinez L, Torre-Cisneros J. Ceftazidime-avibactam in the treatment of infections caused by KPC-producing Klebsiella pneumoniae: factors associated with clinical efficacy in a single-center cohort. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. 2020. 56 (3):- DOI: 10.1016/j.ijantimicag.2020.106075 
IF: 4,621 Q: 1

Larrosa MN, Benito N, Canton R, Canut A, Cercenado E, Fernandez-Cuenca F, Guinea J, Lopez-Navas A, Moreno MA, Oliver A, Martinez-Martinez L. From CLSI to EUCAST, a necessary step in Spanish laboratories. ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA. 2020. 38 (2):79-83 DOI: 10.1016/j.eimc.2018.09.014 
IF: 1,654 Q: 4

Bleriot I, Trastoy R, Blasco L, Fernandez-Cuenca F, Ambroa A, Fernandez-Garcia L, Perez-Nadales E, Torre-Cisneros J, Oteo-Iglesias J, Navarro F, Miro E, Pascual A, Bou G, Martinez-Martinez L, Tomas M. Genomic analysis of 40 prophages located in the genomes of 16 carbapenemase-producing clinical strains of Klebsiella pneumoniae. MICROBIAL GENOMICS. 2020. 6 (5):- DOI: 10.1099/mgen.0.000369 
IF: 4,632 Q: 1 D: 1

Gamal D, Egea P, Elias C, Fernandez-Martinez M, Causse M, Perez-Nadales E, Salem D, Fam N, Diab M, Abu Aitta A, El-Defrawy I, Martinez-Martinez L. High-risk clones and novel sequence type ST4497 of Klebsiella pneumoniae clinical isolates producing different alleles of NDM-type and other carbapenemases from a single tertiary-care centre in Egypt. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. 2020. 56 (6):- DOI: 10.1016/j.ijantimicag.2020.106164 
IF: 4,621 Q: 1

Rivera-Espinar F, Machuca I, Tejero R, Rodriguez J, Mula A, Marfil E, Cano A, Gutierrez-Gutierrez B, Rodriguez M, Pozo JC, De la Fuente C, Rodriguez-Bano J, Martinez-Martinez L, Leon R, Torre-Cisneros J. Impact of KPC Production and High-Level Meropenem Resistance on All-Cause Mortality of Ventilator-Associated Pneumonia in Association with Klebsiella pneumoniae. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2020. 64 (6):- DOI: 10.1128/AAC.02164-19 
IF: 4,904 Q: 1

Blasco L, Ambroa A, Trastoy R, Bleriot I, Moscoso M, Fernandez-Garcia L, Perez-Nadales E, Fernandez-Cuenca F, Torre-Cisneros J, Oteo-Iglesias J, Oliver A, Canton R, Kidd T, Navarro F, Miro E, Pascual A, Bou G, Martinez-Martinez L, Tomas M. In vitro and in vivo efficacy of combinations of colistin and different endolysins against clinical strains of multi-drug resistant pathogens. SCIENTIFIC REPORTS. 2020. 10 (1):- DOI: 10.1038/s41598-020-64145-7 
IF: 3,998 Q: 1

Villalba M, Linares-Sicilia MJ, Gonzalez-Andrades M, Eisman M, Gimenez-Almenara J, Arias M, Villalba R. In vitro susceptibility of microorganisms isolated from cold stored corneas: increased gentamicin-resistance in cornea banking. CELL AND TISSUE BANKING. 2020. 21 (1):159-165 DOI: 10.1007/s10561-020-09811-w 
IF: 1,149 Q: 4

Valle-Arroyo J, Aguado R, Paez-Vega A, Perez AB, Gonzalez R, Fornes G, Torre-Cisneros J, Cantisan S. Lack of cytomegalovirus (CMV)-specific cell-mediated immune response using QuantiFERON-CMV assay in CMV-seropositive healthy volunteers: fact not artifact. SCIENTIFIC REPORTS. 2020. 10 (1):- DOI: 10.1038/s41598-020-64133-x 
IF: 3,998 Q: 1

Romero I, Martinez-Medina M, Camprubi-Font C, Bennour I, Moreno D, Martinez-Martinez L, Teixidor F, Fox MA, Vinas C. Metallacarborane Assemblies as Effective Antimicrobial Agents, Including a Highly Potent Anti-MRSA Agent. ORGANOMETALLICS. 2020. 39 (23):4253-4264 DOI: 10.1021/acs.organomet.0c00315 
IF: 3,804 Q: 1

Perez-Nadales E, Gutierrez-Gutierrez B, Natera AM, Abdala E, Magalhaes MR, Mularoni A, Monaco F, Pierrotti LC, Freire MP, Iyer RN, Steinke SM, Calvi EG, Tumbarello M, Falcone M, Fernandez-Ruiz M, Costa-Mateo JM, Rana MM, Strabelli TMV, Paul M, Farinas MC,. Predictors of mortality in solid organ transplant recipients with bloodstream infections due to carbapenemase-producing Enterobacterales: The impact of cytomegalovirus disease and lymphopenia. AMERICAN JOURNAL OF TRANSPLANTATION. 2020. 20 (6):1629-1641 DOI: 10.1111/ajt.15769 
IF: 7,338 Q: 1 D: 1

Canton R, Oliver A, Alos JI, de Benito N, Bou G, Campos J, Calvo J, Canut A, Castillo J, Cercenado E, Dominguez MA, Fernandez-Cuenca F, Guinea J, Larrosa N, Linares J, Lopez-Cerero L, Lopez-Navas A, Marco F, Mirelis B, Moreno-Romo MA, Morosini MI, Navarro. Recommendations of the Spanish Antibiogram Committee (COESANT) for selecting antimicrobial agents and concentrations for in vitro susceptibility studies using automated systems. ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA. 2020. 38 (4):182-187 DOI: 10.1016/j.eimc.2019.01.017 
IF: 1,654 Q: 4

de Salazar A, Aguilera A, Trastoy R, Fuentes A, Alados JC, Causse M, Galan JC, Moreno A, Trigo M, Perez-Ruiz M, Roldan C, Pena MJ, Bernal S, Serrano-Conde E, Barbeito G, Torres E, Riazzo C, Cortes-Cuevas JL, Chueca N, Coira A, Sanchez-Calvo JM, Marfil E, . Sample pooling for SARS-CoV-2 RT-PCR screening. CLINICAL MICROBIOLOGY AND INFECTION. 2020. 26 (12):- DOI: 10.1016/j.cmi.2020.09.008 
IF: 7,117 Q: 1 D: 1

Vaquero-Alvarez E, Cubero-Atienza A, Ruiz-Martinez P, Vaquero-Abellan M, Redel-Macias MD, Aparicio-Martinez P. Tuberculosis and Other Airborne Microbes in Occupational Health and Safety. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH. 2020. 17 (19):- DOI: 10.3390/ijerph17197088 
IF: 2,849 Q: 1

Cano A, Guzman-Puche J, Garcia-Gutierrez M, Caston JJ, Gracia-Ahufinger I, Perez-Nadales E, Recio M, Natera AM, Marfil-Perez E, Martinez-Martinez L, Torre-Cisneros J. Use of carbapenems in the combined treatment of emerging ceftazidime/avibactam-resistant and carbapenem-susceptible KPC-producing Klebsiella pneumoniae infections: Report of a case and review of the literature. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE. 2020. 22 ():9-12 DOI: 10.1016/j.jgar.2019.11.007 
IF: 2,706 Q: 2

Sanchez-Diener I, Zamorano L, Pena C, Ocampo-Sosa A, Cabot G, Gomez-Zorrilla S, Almirante B, Aguilar M, Granados A, Calbo E, Rodriguez-Bano J, Rodriguez-Lopez F, Tubau F, Martinez-Martinez L, Navas A, Oliver A. Weighting the impact of virulence on the outcome of Pseudomonas aeruginosa bloodstream infections. CLINICAL MICROBIOLOGY AND INFECTION. 2020. 26 (3):351-357 DOI: 10.1016/j.cmi.2019.06.034 
IF: 7,117 Q: 1

Rocha DJP, Azevedo V, Brenig B, Silva A, Blom J, Ramos RT, Aguiar ERG, Chapartegui-Gonzalez I, Fernandez-Martinez M, Martinez-Martinez L, Pacheco LGC, Navas J. Whole-genome sequencing reveals misidentification of a multidrug-resistant urine clinical isolate as Corynebacterium urealyticum. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE. 2020. 23 ():16-19 DOI: 10.1016/j.jgar.2020.07.020 
IF: 2,706 Q: 2

Fernandez-Cuenca, F; Martinez-Martinez, L; Pascual, A. Evolution of the antimicrobial resistance rates in clinical isolates of Pseudomonas aeruginosa causing invasive infections in the south of Spain.. ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA. 2020. 38 (4):150-154 DOI: 10.1016/j.eime.2019.06.009 
IF: 1,654 Q: 4

Publications in 2019

Palacios-Baena ZR, Delgado-Valverde M, Mendez AV, Almirante B, Gomez-Zorrilla S, Borrell N, Corzo JE, Gurgui M, de la Calle C, Garcia-Alvarez L, Ramos L, Gozalo M, Morosini MI, Molina J, Causse M, Pascual A, Rodriguez-Bano J, de Cueto M, Reig AMP, Quintan; Impact of De-escalation on Prognosis of Patients With Bacteremia due to Enterobacteriaceae: A Post Hoc Analysis From a Multicenter Prospective Cohort. CLINICAL INFECTIOUS DISEASES. 2019 Aug 30;69(6):956-962. doi: 10.1093/cid/ciy1032.
IF: 9,055 D: 1 

Perez AB, Vrancken B, Chueca N, Aguilera A, Reina G, Toro MG, Vera F, von Wichman MA, Arenas JI, Tellez F, Pineda JA, Omar M, Bernal E, Rivero-Juarez A, Fernandez-Fuertes E, de la Iglesia A, Pascasio JM, Lemey P, Garcia F, Cuypers L; Increasing importance of European lineages in seeding the hepatitis C virus subtype 1a epidemic in Spain. EUROSURVEILLANCE. 2019 Feb;24(9). doi: 10.2807/1560-7917.ES.2019.24.9.1800227.
IF: 7,421 D1 

Kieffer N, Guzman-Puche J, Poirel L, Kang HJ, Jeon CO, Nordmann P; ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2019 Jun 1;74(6):1568-1571. doi: 10.1093/jac/dkz057.
IF: 5,217 D: 1 

Rodriguez-Gomez J, Perez-Nadales E, Gutierrez-Gutierrez B, Machuca I, Martinez-Martinez L, Rivera F, Cano A, Caston JJ, Robles JC, de la Fuente C, Rodriguez-Lopez F, Rodriguez-Bano J, Torre-Cisneros J; Prognosis of urinary tract infection caused by KPC-producing Klebsiella pneumoniae: The impact of inappropriate empirical treatment. JOURNAL OF INFECTION. 2019 Sep;79(3):245-252. doi: 10.1016/j.jinf.2019.06.014. 
IF: 5,099 Q: 1
 
del Barrio-Tofino E, Zamorano L, Cortes-Lara S, Lopez-Causape C, Sanchez-Diener I, Cabot G, Bou G, Martinez-Martinez L, Oliver A, Galan F, Gracia I, Rodriguez MA, Martin L, Sanchez JM, Vinuela L, Garcia MV, Lepe JA, Aznar J, Lopez-Hernandez I, Seral C, Ca; Spanish nationwide survey on Pseudomonas aeruginosa antimicrobial resistance mechanisms and epidemiology. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 72019 Jul 1;74(7):1825-1835. doi: 10.1093/jac/dkz147.
IF: 5,113 D: 1  

Castro C, Galan-Sanchez F, Linares MJ, Tejero R, Ruiz M, Serrano ML, Rodriguez-Iglesias M, Martin-Mazuelos E, Mediavilla C, Clavijo-Frutos E, Dominguez A, Fajardo M, Freyre C, Galan F, Garcia F, Linares MJ, Lopez-Prieto MD, Martin-Mazuelos E, Navarro JM, ; A prospective survey of Aspergillus spp. in respiratory tract samples: Species identification and susceptibility patterns. MEDICAL MYCOLOGY. 2019 Jun 1;57(4):412-420. doi: 10.1093/mmy/myy080.
IF: 2,851 D: 1   

Perez-Vazquez M, Oteo-Iglesias J, Sola-Campoy PJ, Carrizo-Manzoni H, Bautista V, Lara N, Aracil B, Alhambra A, Martinez-Martinez L, Campos J; Characterization of Carbapenemase-Producing Klebsiella oxytoca in Spain, 2016-2017. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2019 May 24;63(6). pii: e02529-18. doi: 10.1128/AAC.02529-18
IF: 4,715 Q: 1 

Ruiz P, Causse M, Vaquero M, Casal M; In Vitro Activity of Tedizolid against Mycobacterium tuberculosis. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2019 Mar 27;63(4). pii: e01939-18. doi: 10.1128/AAC.01939-18.
IF: 4,715 Q: 1  

Camprubi-Font C, del Castillo BR, Barrabes S, Martinez-Martinez L, Martinez-Medina M; Amino Acid Substitutions and Differential Gene Expression of Outer Membrane Proteins in Adherent-Invasive Escherichia coli. FRONTIERS IN MICROBIOLOGY. 2019 Aug 6;10:1707. doi: 10.3389/fmicb.2019.01707.
IF: 4,259 Q: 1   

Ramos-Vivas J, Chapartegui-Gonzalez I, Fernandez-Martinez M, Gonzalez-Rico C, Fortun J, Escudero R, Marco F, Linares L, Montejo M, Aranzamendi M, Munoz P, Valerio M, Aguado JM, Resino E, Ahufinger IG, Vega AP, Martinez L, Farinas MC, San Millan JCR, Rodri; Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients. SCIENTIFIC REPORTS. 2019 Jun 20;9(1):8928. doi: 10.1038/s41598-019-45060-y.
IF: 4,011 Q: 1 

Ishikawa M, Garcia-Mateo N, Cusak A, Lopez-Hernandez I, Fernandez-Martinez M, Muller M, Ruttiger L, Singer W, Lowenheim H, Kosec G, Fujs S, Martinez-Martinez L, Schimmang T, Petkovic H, Knipper M, Duran-Alonso MB; Lower ototoxicity and absence of hidden hearing loss point to gentamicin C1a and apramycin as promising antibiotics for clinical use. SCIENTIFIC REPORTS. 2019 Feb 20;9(1):2410. doi: 10.1038/s41598-019-38634-3.
IF: 4,011 Q: 1  

Perez AB, Chueca N, Macias J, Pineda JA, Salmeron J, Rivero-Juarez A, Hidalgo-Tenorio C, Espinosa MD, Tellez F, Von-Wichmann MA, Omar M, Santos J, Hernandez-Quero J, Anton JJ, Collado A, Lozano AB, Garcia-Deltoro M, Casado M, Pascasio JM, Selfa A, Rosales; Prevalence of resistance associated substitutions and efficacy of baseline resistance-guided chronic hepatitis C treatment in Spain from the GEHEP-004 cohort. PLOS ONE. 2019 Aug 30;14(8):e0221231. doi: 10.1371/journal.pone.0221231. 
IF: 3,776 Q: 2

Blasco L, Ambroa A, Lopez M, Fernandez-Garcia L, Bleriot I, Trastoy R, Ramos-Vivas J, Coenye T, Fernandez-Cuenca F, Vila J, Martinez-Martinez L, Rodriguez-Bano J, Pascual A, Cisneros JM, Pachon J, Bou G, Tomas M; Combined Use of the Ab105-2 phi Delta CI Lytic Mutant Phage and Different Antibiotics in Clinical Isolates of Multi-Resistant Acinetobacter baumannii. MICROORGANISMS. 2019 Nov 12;7(11). pii: E556. doi: 10.3390/microorganisms7110556.
IF: 4,167 Q: 2

Puig CRD, Torres MF, Marfil-Perez E, Ferandez MIR, Del Rio MC, Balbin JA, Martinez-Martinez L; Comparison between Vitek MS, Bruker Biotyper, Vitek2, and API20E for differentiation of species of the genus Raoultella. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. 2019 Mar;38(3):467-470. doi: 10.1007/s10096-018-03444-4. 
IF: 2,591 Q: 3  

Paez-Vega A, Cantisan S, Vaquero JM, Vidal E, Luque-Pineda A, Lobo-Acosta MA, Perez AB, Alonso-Moralejo R, Iturbe D, Monforte V, Otero-Gonzalez I, Pastor A, Ussetti P, Torre-Cisneros J; Efficacy and safety of the combination of reduced duration prophylaxis followed by immuno-guided prophylaxis to prevent cytomegalovirus disease in lung transplant recipients (CYTOCOR STUDY): an open-label, randomised, non-inferiority clinical trial. BMJ OPEN. 2019 Aug 15;9(8):e030648. doi: 10.1136/bmjopen-2019-030648.
IF: 2,376 Q: 2

Lopez-Camacho E, Pano-Pardo JR, Sotillo A, Elias-Lopez C, Martinez-Martinez L, Gomez-Gil R, Mingorance J; Meropenem heteroresistance in clinical isolates of OXA-48-producing Klebsiella pneumoniae. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. 2019 Feb;93(2):162-166. doi: 10.1016/j.diagmicrobio.2018.09.008. 
IF: 2,314 Q: 3 

Haeili M, Kafshdouz M, Pishnian Z, Feizabadi MM, Martinez-Martinez L; Comparison of susceptibility testing methods for determining the activity of colistin against Gram-negative bacilli of clinical origin. JOURNAL OF MEDICAL MICROBIOLOGY. 2019 Jan;68(1):60-66. doi: 10.1099/jmm.0.000879.
IF: 1,926 Q: 4

Martinez-Martinez L; Carbapenemases: The never-ending story. ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA. 2019 Feb;37(2):73-75. doi: 10.1016/j.eimc.2018.12.006.
IF: 1,685 Q: 4   

Guzman-Puche J, Gracia-Ahufinger I, Causse M, Tejero-Garcia R, Rodriguez-Lopez FC, Casal-Roman M; System, gram-stain and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for diagnosis of urinary tract infections directly from urine samples. JOURNAL OF CHEMOTHERAPY. 2019 Apr;31(2):74-80. doi: 10.1080/1120009X.2019.1573284. 
IF: 1,599 Q: 4 

Cirit OS, Fernandez-Martinez M, Yayla B, Martinez-Martinez L; Aminoglycoside resistance determinants in multiresistant Escherichia coli and Klebsiella pneumoniae clinical isolates from Turkish and Syrian patients. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA. 2019 Sep 1;66(3):327-335. doi: 10.1556/030.66.2019.005.
IF: 1,107 Q: 4   

Pérez AB, Chueca N, García-Deltoro M, Martínez-Sapiña AM, Lara-Pérez MM, García-Bujalance S, Aldámiz-Echevarría T, Vera-Méndez FJ, Pineda JA, Casado M, Pascasio JM, Salmerón J, Alados-Arboledas JC, Poyato A, Téllez F, Rivero-Juárez A, Merino D, Vivancos-Gallego MJ, Rosales-Zábal JM, García F; GEHEP-004 Study Group. High efficacy of resistance-guided retreatment of HCV patients failing NS5A inhibitors in the real world. JOURNAL OF HEPATOLOGY. 2019 Nov;71(5):876-888. doi: 10.1016/j.jhep.2019.06.022. 

Del Barrio-Tofiño E, Sánchez-Diener I, Zamorano L, Cortes-Lara S, López-Causapé C, Cabot G, Bou G, Martínez-Martínez L, Oliver A; GEMARA-SEIMC/REIPI. Association between Pseudomonas aeruginosa O-antigen serotypes, resistance profiles and high-risk clones: results from a Spanish nationwide survey. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2019 Nov 1;74(11):3217-3220. doi: 10.1093/jac/dkz346.

Publications in 2018

Cano A, Gutierrez-Gutierrez B, Machuca I, Gracia-Ahufinger I, Perez-Nadales E, Causse M, Caston JJ, Guzman-Puche J, Torre-Gimenez J, Kindelan L, Martinez-Martinez L, Rodriguez-Bano J, Torre-Cisneros J. Risks of Infection and Mortality Among Patients Colonized With Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae: Validation of Scores and Proposal for Management. CLINICAL INFECTIOUS DISEASES. 2018; 66(8):1204-1210.
IF: 9,117 Q: 1 D: 1

Amat T, Gutierrez-Pizarraya A, Machuca I, Gracia-Ahufinger I, Perez-Nadales E, Torre-Gimenez A, Garnacho-Montero J, Cisneros JM, Torre-Cisneros J. The combined use of tigecycline with high-dose colistin might not be associated with higher survival in critically ill patients with bacteraemia due to carbapenem-resistant Acinetobacter baumannii. CLINICAL MICROBIOLOGY AND INFECTION. 2018; 24(6):630-634.
IF: 5,394 Q: 1 D: 1

Hamzaoui Z, Ocampo-Sosa A, Martinez MF, Landolsi S, Ferjani S, Maamar E, Saidani M, Slim A, Martinez-Martinez L, Boutiba-Ben Boubaker I. Role of association of OmpK35 and OmpK36 alteration and bla(ESBL) and/or bla(AmpC) genes in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. 2018; 52(6):898-905.
IF: 4,253 Q: 1

Aguirre-Quinonero A, del Molino ICP, de la Fuente CG, Sanjuan MC, Aguero J, Martinez-Martinez L. Phenotypic detection of clinical isolates of Haemophilus influenzae with altered penicillin-binding protein 3. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. 2018; 37(8):1475-1480.
IF: 2,537 Q: 2

Fernandez-Martinez M, del Castillo BR, Lecea-Cuello MJ, Rodriguez-Bano J, Pascual A, Martinez-Martinez L. Prevalence of Aminoglycoside-Modifying Enzymes in Escherichia coli and Klebsiella pneumoniae Producing Extended Spectrum -Lactamases Collected in Two Multicenter Studies in Spain. MICROBIAL DRUG RESISTANCE. 2018; 24(4):367-376.
IF: 2,344 Q: 3

Gutierrez-Aroca JB, Ruiz P, Vaquero M, Causse M, Casal M. Surveillance of Drug-Resistant Tuberculosis in Spain (2001-2015). Microb Drug Resist 2018; 24:839-843.

Gutierrez-Aroca JB, Ruiz-Martinez P, Rodriguez-Ruiz L, Casal-Roman M. Distribution of hepatitis C genotypes in the city of Cordoba. Rev Esp Quimioter 2018; 31:164-165.

Espinel-Ingroff A, Turnidge J, Alastruey-Izquierdo A, Dannaoui E, Garcia-Effron G, Guinea J, Kidd S, Pelaez T, Sanguinetti M, Meletiadis J, Botterel F, Bustamante B, Chen YC, Chakrabarti A, Chowdhary A, Chryssanthou E, Cordoba S, Gonzalez GM, Guarro J, Johnson EM, Kus JV, Lass-Florl C, Linares-Sicilia MJ, Martin-Mazuelos E, Negri CE, Pfaller MA, Tortorano AM. Posaconazole MIC Distributions for Aspergillus fumigatus Species Complex by Four Methods: Impact of cyp51A Mutations on Estimation of Epidemiological Cutoff Values. Antimicrob Agents Chemother 2018: 62: e01916-17.

Espinel-Ingroff A, Turnidge J, Alastruey-Izquierdo A, Botterel F, Canton E, astro C, Chen YC, Chen Y, Chryssanthou E, Dannaoui E, Garcia-Effron G, Gonzalez  GM, Govender NP, Guinea J, Kidd S, Lackner M, Lass-Flörl C, Linares-Sicilia MJ, López-Soria L, Magobo R, Pelaez T, Quindós G, Rodriguez-Iglesia MA, Ruiz MA, Sánchez-Reus F, Sanguinetti M, Shields R, Szweda P, Tortorano A, Wengenack NL, Bramati S, Cavanna C, DeLuca C, Gelmi M, Grancini A, Lombardi G, Meletiadis J, Negri CE, Passera M, Peman J, Prigitano A, Sala E, Tejada M. Method-dependent epidemiological cutoff values (ECVs) for detection of triazole resistance in Candida and Aspergillus species for the SYO colorimetric broth and Etest agar diffusion methods. Antimicrob Agents Chemother 2019, 63: pii: AAC.01651-18.

Alastruey-Izquierdo A, Alcazar-Fuoli L, Rivero-Menendez O, Ayats J, Castro C, Garcia-Rodriguez J, Goterris-Bonet L, Ibanez-Martinez E, Linares-Sicilia MJ, Martin-Gomez MT, Martin-Mazuelos E, Pelaez T, Peman J, Rezusta A, Rojo S, Tejero R, Anza DV, Vinuelas J, Zapico MS, Cuenca-Estrella M. Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study). Antimicrob Agents Chemother 2018: 62: e00358-18.

Garcia-Castillo M, Garcia-Fernandez S, Gomez-Gil R, Pitart C, Oviano M, Gracia-Ahufinger I, Diaz-Reganon J, Tato M, Canton R. Activity of ceftazidime-avibactam against carbapenemase-producing Enterobacteriaceae from urine specimens obtained during the infection-carbapenem resistance evaluation surveillance trial (iCREST) in Spain. Int J Antimicrob Agents 2018, 51:511-515.

Lopez M, Rueda A, Florido JP, Blasco L, Fernandez-Garcia L, Trastoy R, Fernandez-Cuenca F, Martinez-Martinez L, Vila J, Pascual A, Bou G, Tomas M. Evolution of the Quorum network and the mobilome (plasmids and bacteriophages) in clinical strains of Acinetobacter baumannii during a decade. Sci Rep 2018, 8:2523.

Zboromyrska Y, Bosch J, Aramburu J, Cuadros J, Garcia-Riestra C, Guzman-Puche J, Martos CL, Loza E, Munoz-Algarra M, de Alegria CR, Sanchez-Hellin V, Vila J. A multicentre study investigating parameters which influence direct bacterial identification from urine. Plos One. 2018: 13: Núm artículo e0207822.

Fernandez-Garcia L, Ambroa A, Blasco L, Bleriot I, Lopez M, Alvarez-Marin R, Fernandez-Cuenca F, Martinez-Martinez L, Vila J, Rodriguez-Bano J, Gamacho-Montero J, Cisneros JM, Pascual A, Pachon J, Bou G, Smani Y, Tomas M. Relationship Between the Quorum Network (Sensing/Quenching) and Clinical Features of Pneumonia and Bacteraemia Caused by A. baumannii. Front Microbiol 2018; 9: 3105

Cerezales M, Ocampo-Sosa AA, Montes LA, Rios CD, Bustamante Z, Santos J, Martinez-Martinez L, Higgins PG, Gallego L. High Prevalence of Extensively Drug-resistant Acinetobacter baumannii at a Children Hospital in Bolivia. Ped Infect Dis J 2018, 37:1118-1123.

Aguado JM, Silva JT, Fernandez-Ruiz M, Cordero E, Fortun J, Gudiol C, Martinez-Martinez L, Vidal E, Almenar L, Almirante B, Canton R, Carratala J, Caston JJ, Cercenado E, Cervera C, Cisneros JM, Crespo-Leiro MG, Cuervas-Mons V, Elizalde-Fernandez J, Farinas MC, Gavalda J, Goyanes MJ, Gutierrez-Gutierrez B, Hernandez D, Len O, Lopez-Andujar R, Lopez-Medrano F, Martin-Davila P, Montejo M, Moreno A, Oliver A, Pascual A, Perez-Nadales E, Roman-Broto A, San-Juan R, Seron D, Sole-Jover A, Valerio M, Munoz P, Torre-Cisneros J. Management of multidrug resistant Gram-negative bacilli infections in solid organ transplant recipients: SET/GESITRA-SEIMC/REIPI recommendations. Transplant Rev 2018, 32:36-57.

Cercenado E, Canton R, Ramirez MG, Mansilla CA, Ahufinger IG, Ramirez MG, Alarcon JM. Procedures in Clinical Microbiology (number 15.(a), 2nd edition 2018). Enf Infec Microbiol Clin 2018, 36:603-603.

Rodriguez-Lozano J, de Malet A, Cano ME, de la Rubia L, Wallmann R, Martinez-Martinez L, Calvo J. Antimicrobial susceptibility of microorganisms that cause urinary tract infections in pediatric patients. Enf Infec Microbiol Clin 2018, 36:417-422.

Publications in 2017

Delgado-Valverde M, Valiente-Mendez A, Torres E, Almirante B, Gomez-Zorrilla S, Borrell N, Aller-Garcia AI, Gurgui M, Almela M, Sanz M, Bou G, Martinez-Martinez L, Canton R, Lepe JA, Causse M, Gutierrez-Gutierrez B, Pascual A, Rodriguez-Bano J, REIPI/GEIH-SEIMC BACTERAEMIA-MIC Group. MIC of amoxicillin/clavulanate according to CLSI and EUCAST: discrepancies and clinical impact in patients with bloodstream infections due to Enterobacteriaceae. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2017.72(5):1478-1487.
IF: 5,071 Q: 1 

del Barrio-Tofino E, Lopez-Causape C, Cabot G, Rivera A, Benito N, Segura C, Montero MM, Sorli L, Tubau F, Gomez-Zorrilla S, Tormo N, Dura-Navarro R, Viedma E, Resino-Foz E, Fernandez-Martinez M, Gonzalez-Rico C, Alejo-Cancho I, Martinez JA, Labayru-Echverria C, Duenas C, Ayestaran I, Zamorano L, Martinez-Martinez L, Horcajada JP, Oliver A. Genomics and Susceptibility Profiles of Extensively Drug-Resistant Pseudomonas aeruginosa Isolates from Spain. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2017.61(11):-e01589-17.
IF: 4,302 Q: 1 

Sanchez-Diener I, Zamorano L, Lopez-Causape C, Cabot G, Mulet X, Pena C, del Campo R, Canton R, Domenech-Sanchez A, Martinez-Martinez L, Arcos SC, Navas A, Oliver A. Interplay among Resistance Profiles, High-Risk Clones, and Virulence in the Caenorhabditis elegans Pseudomonas aeruginosa Infection Model. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2017.61(12):-e01586-17.
IF: 4,302 Q: 1 

Lazaro-Diez M, Chapartegui-Gonzalez I, Redondo-Salvo S, Leigh C, Merino D, Segundo DS, Navas J, Icardo JM, Acosta F, Ocampo-Sosa A, Martinez-Martinez L, Ramos-Vivas J. Human neutrophils phagocytose and kill Acinetobacter baumannii and A-pittii. SCIENTIFIC REPORTS. 2017.7(1):-4571.
IF: 4,259 Q: 1 

Alibi S, Ferjani A, Boukadida J, Cano ME, Fernandez-Martinez M, Martinez-Martinez L, Navas J. Occurrence of Corynebacterium striatum as an emerging antibiotic-resistant nosocomial pathogen in a Tunisian hospital. SCIENTIFIC REPORTS. 2017.7():-9704.
IF: 4,259 Q: 1 

Machuca I, Gutierrez-Gutierrez B, Gracia-Ahufinger I, Espinar FR, Cano A, Guzman-Puche J, Perez-Nadales E, Natera C, Rodriguez M, Leon R, Caston JJ, Rodriguez-Lopez F, Rodriguez-Bano J, Torre-Cisneros J. Mortality Associated with Bacteremia Due to Colistin-Resistant Klebsiella pneumoniae with High-Level Meropenem Resistance: Importance of Combination Therapy without Colistin and Carbapenems. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2017.61(8):-e00406-17.
IF: 4,302 Q: 1 

Ruiz P, Causse M, Vaquero M, Gutierrez JB, Casal M. Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis. INFECTION AND DRUG RESISTANCE. 2017.10():463-467.
IF: 3,779 Q: 1 

Gonzalez-Garcia FJ, Guzman-Puche J, Redel-Montero J, Moreno P. Fatal bilateral haemopneumothoraces following double-lung transplantation caused by pretransplant colonization by Scedosporium apiospermum. EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY. 2017.51(6):1209-1209.
IF: 3,759 Q: 2

Clinical Research

Active in 2018

Intestinal colonization by multiresistant enterobacteria in patients with renal and liver transplant: multicenter cohort study.
PI: Dr Irene Gracia Ahufinger

Impact of intestinal colonization by multiresistant enterobacteria in systemic infections, GVHD and mortality of patients receiving allogeneic bone marrow transplantation.
PI: Dr Irene Gracia Ahufinger

Registro de infecciones por bacterias. MULTIRRESISTENTES_SEIMC_2018.
PI: Dr Irene Gracia Ahufinger

Members

Principal Investigator
Luis Martínez Martínez

Researchers
Manuel Causse Del Río
María del Pilar Egea Miranda
Irene Gracia Ahufinger
María José Linares Sicilia
Ana Belén Pérez Jiménez
Fernando Carlos Rodríguez López
Pilar Ruiz Martínez
Rocío Tejero García

Post-Doctoral Researchers
María del Mar Bellido Barquero

Pre-Doctoral Researchers
Luis Ayala Morillo
Tania Blanco Martín
Clara de Asís Morales Domínguez
Mónica Espinar García
Julia Guzmán Puche
Eduardo Marfil Pérez
Montserrat Muñoz de la Rosa
Rosa Pedraza Merino
Irene Sánchez León
Cristina Riazzo Damas

Nursing, Technical, and Administrative Staff
María Córdoba Fernández
Cristina Elías López